The end product DNA pellet is gel like and will not re suspend nor will it load it a agarose gel. Any suggestions on how to avoid this or what may be causing the problem?
formation of gel due to polysaccharide you can remove by sorbitol buffer to remove mucilaginous polysaccharides
http://nopr.niscair.res.in/bitstream/123456789/13514/1/IJBT%2011(1)%2067-71.pdf
Dear Denise, the link and attached file can help you.
Best regards.
https://www.researchgate.net/publication/21651496_A_quick_and_inexpensive_method_for_removing_polysaccharides_from_plant_genomic_DNA
Article A quick and inexpensive method for removing polysaccharides ...
One possibility is the coextraction and precipitation of sugars with your DNA, right, but in addition to that, also can be alcohol left, if you use it in the previous precipitation. This results in a DNA solution that even when mixed with loading buffer, has lower density than TBE or TAE running buffer, thus not loading on the gel wells. If so, put your DNA tubes open at 60ºC degrees for 10 minutes to evaporate the ethanol.
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