I'm looking for a protocol how to isolate gDNA from pet fur/hair obtained after brushing. I know it will only contain very few nucleated cells. Anyone? Preferably without using phenol/chloroform.
I won't need much, just enough to do 5-10 PCRs.
Any advice, suggestion or reference would be greatly appreciated.
The reason I would like to know is so we can minimize stress for cats when obtaining their DNA. A cheek swab is possible, but would be more stressful.
https://www.ncbi.nlm.nih.gov/pubmed/15062955
https://investigativegenetics.biomedcentral.com/articles/10.1186/2041-2223-5-7
You could try the chelex method mentioned in this paper: "Highly reliable genetic identification of individual northern hairy-nosed wombats from single remotely collected hairs: a feasible censusing method." Good luck!
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