DNA Extraction of Pedicularis - dark brown after elution in TE?

Kas Molina @Kas-Molina

30 May 2019 0 6K Report

I am using a CTAB/Magnetic Beads protocol to obtain genomic DNA from Pedicualris (plant)

I homogenize, add CTAB & Proteinase k

Incubate for 30 at 55C

Add Sodium acetate

Incubate at 4C for 30

Centrifuge and keep supernatant (pellet in this protocol in "junk")

Add beads & PEG - incubate a room temp

magnetize and wash 3 times with etahnol-isopropanol-ethanol

At the last stage samples look clear, after elution in TE samples become dark brown.

What adjustments could I try?

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