The samples we have are from leaves but dried completely at dark and in closed room. We have followed several extraction steps that included kit, kit with PVP and CTAB. However, the thing that we confront always is purification problems because the samples are not pure enough. Sequentially, this is our very first time to extract DNA from this plant. Any idea what to do? Thanks.
What do you mean not pure enough, are you able to get enough DNA ? But DNA is not working downstream for restriction digest /PCR or not getting DNA at all ?
Dear Yavuz,
I would like to know if you have some good news about the extraction. I am working on E. milii latex as a molluscicide component and this expertise could be important in the future.
Have you published or find some information about this approach?
Best regards,
Ronaldo
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