I am doing DNA separation in micro-fluidic channel network made of glass. There I see DNA sticking on glass channel wall that impedes DNA movements and eventually clog the channel. After few experimental runs, the micro-fluidic chip dies.
Théo Aspert
Hi Rajesh,
Did you try to coat your chamber with a surfactant or with BSA for example (like 0.1mg/mL for 30min)?
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Jules Lloyd Hammond
As Théo Aspert suggests, I would investigate the use of a surfactant.
Jules
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Rajesh Deb
I have never done this before. But I will surely try it. Thanks a lot. I really appreciate your answer. But one thing I am worried about is using surfactant might modify the glass channel surface and thereby kill electro-osmotic flow inside the glass channel. @Thèo Aspert
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