Hello,
I want to prepare a 5mg/mL tetracycline solution to be administered to ant colonies. I cannot use methanol or ethanol for solubilisation. My attempts with dissolving directly in MQwater resulted in precipitation. Anyone would have tips on how to better dissolve tetracycline hydrochloride?
Thanks
Try to heat it. It's soluble up to 50 mg/mL so 5 mg/mL shouldn't be a problem. However you may see some turbidity after cooling.
Thanks Jean, I saw about heating online but was unsure how much heating. Have you tried it this way? Cheers!
I would not boil it, but go as much as needed until it dissolves completely, with vigorous stirring.
Well though I have no experience with your compound, however, whenever we had to dissolve non-polar in water we dissolved the compound in a very little amount of compatible solvent (eg acetone, alcohol etc) thereafter, it become miscible in water
Hello! Thanks everyone for the answers. I must say 1) I am avoiding organic solvents as this would be quite toxic ( also possibly unappealing) to the ants thus I am trying to stick with water; 2) I have heated the water as suggested and got complete dissolution of the compound at about 80oC.
I will now test it on the ants and see if it is still working (they are infected with Wolbachia, which should eventually disappear) and will post what happened here. Thanks!
Perhaps you could use doxycycline (eg soludox) which is water soluble and belongs to the same group of antibiotics as tetracycline does.
I assure you that concentrated "mother waters" in ethanol is quite applicable for many cases with different insects. You must prepare it with 100-fold concentration and then dissolve in water.
Dear colleagues,
I am happy to see so many suggestions provided here. In fact I was using this thread in approaching other authors who often neglect this relative insolubility of this antibiotic (one of them even told me "as a suspension it should be fine", which is clearly a fallacy).
I will return to such tests in the future where your suggestions will be of great value. Let me summarise what happened in the end, as a feedback to all of you following this thread. I have in the end opted to heat up the water and got complete solubility at 80oC, as mentioned above. However the antibiotic did not work as expected, not sure if because of the protocol or if the infection was somehow resistant! Past 11 weeks of antibiotic administration, the infection in my ants was still the same! Thus I moved to using ampicillin from Sigma which was more readily soluble at room temperature.
Hi Eduardo, thanks for your informative and useful post. I am hoping to inject tetracycline into crinoids, as it fluoresces and has been used to mark skeletal growth in echinoids. Did you have to keep the solution at 80 C when administering it to your ants? My crinoids are living at 10 C right now, and I worry that administering such a hot solution would be very shocking for them. Thanks!
Hello, I am sorry as I have been away from this thread. My commments below.
@James: after heating up the solution and dissolving the compound I leftt it cooling down to room temperature and saw no significant precipitation whilst the colour maintained. Moreover delivering to the ants hot would be unpractical and not reasonable.
@ Farhanullah: no I haven't. It would be nice to hear insights into that possibility. I have no experience with emulsifiers.
Hello everyone
Hope you all are experienced persons here, i want to know that whether Doxorubicin.HCl is more soluble or Tetracycline.HCl in PBS buffer.
@Adil majeed Rather -- I have never tried. I suggest you create a dedicated separate question to maximise chances you get the best feedback possible. Good luck!
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