19 Questions 66 Answers 0 Followers
Questions related from Eduardo Goncalves Paterson Fox
I would like to publish one huge table as Supplementary resources to a forthcoming paper. The R package 'plotly' enables the creation of interactive tables which can be searched, filtered for...
05 September 2018 1,544 7 View
I have been separate piperidine alkaloid isomers with ordinary normal phase silica TLC, revealed with Draggendorf's reagent. However now I am attempting some separations using a silver-nitrate...
10 November 2017 6,149 3 View
I am curious at the geographical variation of smell perception towards some ants when they are crushed. Please, if you have these tiny pest insects at home and would be willing to sacrifice few...
20 June 2017 2,683 3 View
Hello everyone, I am preparing a protocol for injecting insects (ant larvae, eggs, lepidopteran larvae) with small volumes such as 30-50nl. I'd be injecting acetone mixed with some oily alkaloids....
10 February 2017 1,046 3 View
Dear collagues, This is probably a very naive question but to date I haven't obtained a satisfactory answer. I often must study old papers. I recurrently find these old papers very reticent and...
12 September 2016 4,732 7 View
Dear colleagues, I am fractionating hexane-extracts of some biological sample, and identifying resulting compounds. Curiously at the end of my extractions, I obtain an oily emulsion which is no...
08 September 2016 4,946 8 View
Hi! I want to help a friend with his bioassays on flies with LED lights. He is using commercial LED lights which come with specifications on nanometers (nm) and intensity of light. However since...
12 August 2016 1,486 13 View
Hi I have just downloaded and installed this (rather complicated) program OpenChrom for OSX. I am using it to see chromatograms and spectra of GC-MS in different formats. I am, however, unable to...
09 August 2016 7,293 4 View
Hello, I want to prepare a 5mg/mL tetracycline solution to be administered to ant colonies. I cannot use methanol or ethanol for solubilisation. My attempts with dissolving directly in MQwater...
02 March 2015 8,456 16 View
I am inexperienced with DAPI or Wolbachia, and I want to use Wolbachia infection as a measure of efficiency of some antibiotic administration to an insect colony. I am afraid of using only PCR as...
15 January 2015 1,388 7 View
I am currently working as a postdoc in Switzerland with fire ants. For obtaining more colonies of fire ants, we must travel far, to the field in subtropical regions. We currently have several...
26 June 2014 2,751 9 View
I would need to do some experiments on ant pupae, but I should genotype them before procedures. As such I would be needing some body part for DNA extraction. I know adults do well after one leg...
06 June 2014 8,625 5 View
Will try to make this question as simple as possible. If anyone has any ideas, please. I am trying to silence a gene in an ant. My target gene for silencing has several spliced transcript forms,...
04 March 2014 4,122 25 View
I am not very experienced with molecular biology, and recently my advisor posed me a difficult question. I will explain as follows. I am comparing relative expression between different genes using...
09 February 2014 4,558 9 View
I am new to HPLC, and I am trying to devise a protocol to deal with poorly studied alkaloids (solenopsins). There is a variety of solvents which can dissolve these compounds, and I am trying to...
21 February 2013 1,126 22 View
I am a user of a Thermo Scientific Surveyor HPLC system with an autosampler. Since we came back from end-of-year festivities vacation, the system is not working anymore, because of unexpected...
14 January 2013 1,566 18 View
Could anyone indicate the best (or most used) software to map stripped ESTs to a predicted transcriptome (or even predicted proteins) from a model organism genome? I would like to build contigs...
15 November 2012 5,377 3 View
I have been having difficulties with a lag between my fraction collector and the cell detector of my Shimadzu HPLC system. I am trying to adjust this delay time by determining the exact time...
12 November 2012 2,571 24 View
I have just started exploring Reddit, and I believe it holds great potential for broadcasting research results and published papers. However, it is not simple to use and there sheer amount of...
01 January 1970 7,000 3 View
