Hi,

I'm looking to use Alomone's ANA-12 as a TrkB receptor antagonist in vitro. It is soluble in DMSO. The goal is to be able to put 100uM in solution with 0.1%DMSO or less due to the toxicity of the DMSO (thus trying to dissolve in as little DMSO as possible). While the Alomone site does not list the solubility ratios, Tocris says that the max concentration is 20mM or 8.15mg/mL. At that dissolution, in order to achieve 100uM, I would wind up with a DMSO ratio of 0.5% in my solution, once it is added to media (5x higher than that recommended for cell culture solutions). I'm not a chemist, but I'm wondering if there are any tweaks I can use to reduce the amount of DMSO used and still get maximal dissolution? Has anyone here used ANA-12 in vitro, and if so, what uM did you use, and did you incorporate any tricks to getting it to dissolve?