In PRIMER, is it possible to downweight certain taxa in multivariate community datasets to reflect uncertainty around identification??

I have a dataset with about 80 different species. As usual, some species are very easy to identify with certainty whereas others are more difficult, which means that I am less certain of my...

03 March 2021 8,066 4 View

How to get onecolumn document from standard IEEE ACCESS LateX template?

I have prepared a manuscript in IEEE Latex template, but for some purpose, I need to prepare a word file as well (detailed format is not required). Compared to two column .tex file, one column...

02 March 2021 1,830 3 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

How to grow E.coli on the low salt LB agar plate with 50 or 100ug/ml of Blasticidin?

Hello, I would like to ask how to grow E.coli which resists 50 or 100ug/ml of Blasticidin. I have tried many methods by using low salt Luria-Bertain(LB) agar plate. However, no colonies grow. Let...

01 March 2021 2,027 2 View

OpenBCI eeg--has anyone used them?

I'm looking to try out an off-the-shelf EEG set for some exploratory work. Curious if anyone has used the OpenBCI, and how much set-up work is required. I've heard they are more difficult to get...

01 March 2021 4,128 3 View

How to remove DCU and HOBT from my reaction?

I am doing a acid and a chiral amino alcohol reaction using DCC and HOBT in DCM or THF? Can anyone suggest me how to remove DCU and HOBT from a reaction mixture??

28 February 2021 2,153 1 View

1 nm thick MgO thin-film to be deposited on Si wafer using thermal evaporation. What will be the optimum deposition conditions?

Favourable conditions of base pressure, rate of deposition. Also, by which characterisation technique to be used to verify the thickness of the deposited film.

28 February 2021 3,640 3 View

How to eliminate Steady state error from Reinforcement learning genetic algorithm code?

The following code (see 1st 2 images attached) is used to produce PID controller values that are designed to control the system (G). The code finds the PID controller values (noted as k) by using...

28 February 2021 6,560 14 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

What is the suitable range of Sulfur content and Viscosity of Heavy fluid Oil that used in Kiln?

What is the disadvantages of high sulfur content to the ceramic burning process? and what is the effect of outranged viscosity?

24 February 2021 8,219 2 View

What is the production process of CaFe ferroalloys?

I looking for ferro calcium production process

03 March 2021 1,755 4 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

Purified protein requires Calcium for activation, but the elution buffer I used seems to interact with Calcium, help?

09 February 2021 6,946 4 View

Phosphate buffer with different pH conditions: HCl or NaH2PO4?

Hi all, I just encountered this dogma; when adjusting pH of phosphate buffers, I've been using HCl/NaOH to in/decrease pH of buffers and recently I was told that HCl/NaOH can't be used to adjust...

08 February 2021 9,070 5 View

Why am I getting such large heats of dilution in my Isothermal Titration Calorimetry Experiment?

I am trying to measure binding of calcium to an expressed protein we are studying and am getting very large heats of dilution for the calcium solution in my control tests. We prepared a 1 M CaCl2...

07 February 2021 7,532 1 View

Why FTIR can not detect bases of nucleic acid in extracellular polymeric substances (EPS)?

I characterized functional groups of EPS using FTIR. Goups of sugars and proteins (e.g., -OH, amide, -COOH) as well as phosphate group of nucleic acid were readily detected. However, peaks of...

05 February 2021 4,405 1 View

Why sometimes current- voltage plot in chemiresistors/ FET does not pass through the origin?

I am trying to measure resistance from I-V curve from a chemiresistor/ FET sensor with a keithly sourcemeter. The substrate is soaked/ wet with protein and phosphate buffer only. But the I-V line...

24 January 2021 8,009 3 View

How can you accurately induce calcium overload and measure membrane potential in cultured Tg2576 neuronal cells using whole cell patch clamp method?

Hi there, I'm currently working on a project where i need to design an experiment but as i have little to no experience with this level of experimental design and no face to face help i am really...

22 January 2021 6,728 3 View

Why calcium oxide nanoparticles becomes black at 650 degree temp?

Please tell me, which temp can I select for neglect black color of the Calcium oxide nanoparticles ..

14 January 2021 5,341 5 View

How do I justify the use of fish scales to synthesize a nano filter?

Good day. I am doing a studu on the use of fish scales to create a nano filter. This is aimed at potentially solving the face mask problem brought about the pandemic. This is what I know so...

09 January 2021 8,907 5 View