i have silk fibroin powder i want to dissolve it in water without adding LiBr or any other dissolving agent how its possible to dossolve in water. if dissolve it at 4C is it possible.
i need to break down its hydrogen bonding then i will be dissolved.
need your kind suggestion.
Dear friend Sakandar Rahman
Now, let's dive into the silk fibroin adventure.
Alright, breaking down the hydrogen bonding in silk fibroin without using LiBr or any other dissolving agent, you Sakandar Rahman say? That's quite a challenge, but I am up for it!
1. **Temperature Matters:**
- Silk fibroin dissolves better in warm water. You Sakandar Rahman might want to try increasing the temperature but be cautious as too much heat could denature the protein.
2. **Mechanical Agitation:**
- Stir, stir, and stir some more! Mechanical agitation can assist in breaking down the hydrogen bonds and aiding dissolution.
3. **Time, Patience, and Persistence:**
- Dissolving silk fibroin without a dissolving agent might take time. Be patient, and let the water work its magic on those hydrogen bonds.
4. **pH Adjustment:**
- Consider adjusting the pH. Silk fibroin is more soluble in water at lower pH values.
5. **Enzymatic Assistance:**
- Proteolytic enzymes might help break down the protein structure. Consider experimenting with enzymes like protease.
6. **Use of Urea or Guanidine Hydrochloride (GuHCl):**
- While you mentioned not wanting to use LiBr, urea or GuHCl could be alternatives. They are known denaturing agents and might aid in silk fibroin dissolution.
7. **Varied Water Sources:**
- Try different types of water. Sometimes the mineral content or other factors in water can influence dissolution.
8. **Additives and Surfactants:**
- In some cases, mild surfactants or additives could be used to aid in the dissolution process.
Regarding temperature, yes, you Sakandar Rahman can try dissolving silk fibroin at 4°C, but keep in mind that generally, warmer temperatures are more conducive. The low temperature might slow down the process.
Now, remember, these are suggestions. Actual success might depend on the specific characteristics of your silk fibroin powder. Don't forget to experiment safely and embrace the adventure of scientific exploration! 🧪
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