Hi, is it alright to use an extension temperature of 68 degrees with GXL enzyme for PCR products below 4kb? Takara (GXL's manufacturer) recommends an extension temperature of 72 degrees with GXL...
03 February 2023 754 12 View
Hi, I am using the same DNA template to run two 7kb PCR experiments with the same cycling conditions simultaneously. The only difference between the two experiments is the primer pair. One result...
02 December 2022 4,813 7 View
Dear Researchers, I would like to know more about how could I consider confounders during my sample size estimation. I had read about controlling confounders using multivariate linear regression...
03 December 2021 5,239 5 View
Hi all! I am currently trying to barcode hepatitis B virus DNA via PCR, but due to the complex nature of HBV DNA, my PCR results have been unsuccessful. Through discussions with my advisor, we...
19 October 2021 3,750 2 View
Dear Sir/ Madam I am currently running a panel data on how Malaysia macroeconomic variables in natural logarithm (CPI, money supply M3, OPR, exchange rate MYR/USD) affect the 5 largest bank stock...
03 July 2021 3,225 8 View
Hi. I am trying to simulate a rapid load test in Plaxis to determine the load transfer mechanism. The load is inputted as a line load and the dynamic multiplier is defined as a table with 1 at the...
22 July 2020 4,968 1 View
Hello, I will be doing protein interaction pull down with the aforementioned kit. I will be using 50ug/100ul of biotinylated peptide as bait. I am wondering how much of the lysate (amount and...
17 June 2020 6,335 0 View
Good day everyone, as mentioned in the title, I would like to ask about the way to determine the tensile properties of each individual layer in a polymeric bilayer film via uniaxial tensile test....
26 May 2020 5,997 6 View
I am currently writing thesis for my undergraduate where i need to design a machine learning model to estimate the soil data between 2 borehole based on the bore log data. Sorry in advance if i...
09 April 2020 398 3 View
I am making a polyurethan film using a ceramic mold and after maturation at 110 Celsius it’s hard for me to strip it off from the ceramic mold. Any chemicals that I should add?
29 December 2019 9,167 3 View
Let's say we have a standard, regular hexagonal honeycomb with a 3-arm primitive unit cell (something like the figure attached; the figure is only representative and not drawn to scale). The...
07 August 2024 1,937 1 View
How can I define dispersion for salt in Eclipse in a low salinity water flooding code? how can I active interactions between tracer and polymer in Eclipse? Please let me know if you have any idea.
05 August 2024 1,218 1 View
How do you tie-break and rank these two in their strength?
03 August 2024 4,471 1 View
I'm trying to calculate the tolerance factors of the hydride perovskite and the value calculated by me and the reported values are contradictory. Need more clarity in using the correct ionic radii...
01 August 2024 2,614 1 View
Dear all: I am modifying Bismuth Tungstate with Hematite (Fe2O3), so I would like to know about how much Iron(+3) can form a solid solution in a Bismuth Oxide matrix. Best wishes Marco
29 July 2024 2,163 1 View
I want to perform DFT calculations for a triple cation mixed crystal. Which software is preferable and free?
28 June 2024 5,255 2 View
i want to do DFT calculations for a triple cation mixed crystal. Can somebody help me in this regard?
27 June 2024 1,804 0 View
If we compare the values, the increase in ΔS0 from AC1 to AC2 (67 J/mol to 143 J/mol) indicates that the amount of adsorption is higher for AC2 compared to AC1. This increase in entropy means that...
10 June 2024 9,287 0 View
since dispersion correction GD3BJ is incompatible with minnesota, which dispersion correction should be added in calculation.
05 June 2024 8,004 1 View
I have recently run several thermal stepping measurements at a temperature range of +20 to -100 °C, at low light intensity (~500 W/m2) on perovskite solar cells based on MAPbI3 and triple-cation...
04 June 2024 3,872 0 View
