Different dyes have certain selectivity for Raman excitation light source. Why?

28 January 2021 1 6K Report

Dyes like MB and CV exhibit Raman enhanced performance under 633 nm excitation light, while RhB and 4-MBA are more suitable for Raman detection under 532 nm excitation light. Is this is because of fluorescence or if some other factor plays a role?

Jürgen Weippert

If your excitation wavelength is close or "on" the absorption band of the dye, you will get resonance Raman:

https://en.wikipedia.org/wiki/Resonance_Raman_spectroscopy

I don't know the absorption for 4-MBA, but RhB has definitely the shorter main absorption wavelength in comparison with MB and CV, so the one that's closer to the absorption wavelength has the stronger enhancement.

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