What is the maximum number of reads per one run with NGS?

Does anybody know how many reads are usually generated with Illumina NextSeq? For sequencing plasmid library (100-150 bp per read), for example.

27 September 2020 799 2 View

Hello dear researchers, how do you remove the black foil from the vine nursery?

Hello, how do you remove the black foil from the vine nursery? Attached picture in the file. is there machine way or by hand?

04 September 2020 3,566 2 View

Glioblastoma GL-261 cell line not growing s.c?

Hi has anyone had experiance of GL-261 not growing subcutaneously? I have inoculated 10e6 cells in C57BL/6J female mice and the tumors became palpable after a week cca but have subsequently...

19 February 2020 7,543 2 View

Microplastic extraction from fish guts using soluble filters?

Hi there, I want to isolate microplastic particles from fish guts and water samples. My initial idea was to extract guts from selected specimens and then dissolve the whole guts using 10% KOH...

12 February 2020 4,467 8 View

Why are my glucose levels decreasing very rapidly?

Hello, I'm conducting an IPGTT experiment on WT and KO mice (mice which we have shown to have improved glucose tolerance) and I have a serious issue of glucose levels plummeting after 30-60min....

28 September 2018 962 4 View

Can you send a code for tweet segmentation?

12 April 2017 8,236 2 View

No title

10 December 2015 1,475 1 View

How can I calculate deflection of steel fiber reinforced concrete beams?

How can I calculate deflection of SFRC beams (ordinary and high strength)? Is there ACI or other proposals?

11 May 2015 4,977 14 View

No title

I need approximately 100 meters of ceramic cord (0,5mm). I need it for attaching extensometer to the tensile specimen when conducting tensile test at elevated temperatures.

10 November 2014 4,572 3 View

What is the best bacterial vector for large inserts (around 50 kb)?

Is it possible to insert 50 Kb of DNA to a bacterial vector and successfully maintain and amplify it in bacteria? If so, what would be the best vector?

12 January 2014 8,832 2 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

What is the production process of CaFe ferroalloys?

I looking for ferro calcium production process

03 March 2021 1,755 4 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View

How to access MSCI KLD Stats and Sustainalytics?

Hello all, I'm currently working on my undergraduate thesis and I'm having difficulties trying to access KLD Stats and Sustainalytics because my campus Airlangga University and apparently other...

02 March 2021 8,525 1 View

Is there any test to determine the cardiovascular risk of patients with obesity? from the perspective of obesity?

We found tests to determine cardiovascular risk taking into account lipid profile, blood pressure, age, history of diabetes, other laboratories. But when weight is involved, there is a paradox. I...

02 March 2021 6,036 1 View