Dear all,
we are trying to perform confocal fluorescence live-cell imaging with the H2S specific fluorescent probe SF-7AM, in bovine aortic endothelial cells. Is there someone expert of this probe? We would like to record time-course variations of H2S, but we don't know the optimal window of time in which the maximal response (to NaSH or other stimuli) occur (seconds or minutes or hours).
Thank you!
Maria Pia