Did my low nitrogen asparagine succinate medium (LN-AS) have gone wrong?

More Aleksandra Gęsiorska's questions See All

What is it? Subject classification codes?

Dear colleagues, help me to understand the rules for authors. I am writing an article for journal "Climacteric". According to the rules for autors it is necessary to indicate "Subject...

09 February 2021 2,221 4 View

How to analyse multiple Likert scales?

Hello everyone! I've encountered a problem when trying to figure out the methodology of my dissertation. The study has multiple variables and will be, in general, rather complicated, but my only...

02 February 2021 2,086 3 View

Variable with negative values in SPSS?

Hello, dear colleagues! I would ask you for a little help. Actually, I'm doing a multivariate linear regression analysis in the SPSS and one of the independent variables has negative values. In...

20 December 2020 535 2 View

ICP-Analysis: how can I consider the loss of sample mass after sample filtration?

To prepare a sample for ICP-Analysis one has to dissolve the material in the chosen liquid and then filter it. How can I consider the loss of mass after filtration if I don't know it exactly? I...

20 December 2020 4,394 3 View

How to report results from piecewise structural equation modelling analysis?

I have done my analysis in R using the piecewiseSEM package and have got the results but I am unsure how to report it APA style. What values do I report? Thank you in advance!

13 December 2020 3,048 3 View

Is USDA NASS plot-level Objective Yield Survey data publicly available?

I'm interested in the results of Objective Yield Survey by USDA NASS in a raw form of plot-level observations. As far, as I understand, quickstats...

19 November 2020 5,369 3 View

What is the most sufficient way of protein immobilization on SiO2?

I need to immobilize 3 peptides: CGPDGRDGRDGRDGR, CRQILIWFQNRRMKWKKYQRLC, penetratin peptide RQIKIWFQNRRMKWKKGG on the SiO2-covered gold nanoparticles. What is the most efficient and stable way...

16 November 2020 2,909 3 View

How to dissolve betulin in DMSO (20mM stock solution)?

I would be very grateful for any help regarding my problem. My research requires applying betulin (and betulinic acid) solution on the cerebral cortex slices of rats. With the stock solution of...

27 September 2020 8,758 2 View

Scanning Electron Microscope- How to correct crossover?

Hello, I have questions about XL30 SEM (with tungsten filament). Can anybody help me with how to correct crossover? You will see in the picture look of my crossover. I trying everything and can't...

24 May 2020 1,339 3 View

Is it possible to precisely evaluate a thickness of a NiP-Cr coating on a stailness steel with XRF?

Hello everybody, I have a qestion about the implementation of the XRF to determine the thickness of the NiP -Cr coating on a stainless steel (1.4305). According to the TDS of the base Material,...

24 February 2020 5,574 2 View

What solvent can I used for Paal Knorr reaction between 6-amino hexanoic acid and 2.5 hexanedione?

I am try to make the Paal Knorr reaction between 2.5 hexnedione and 6-amino hexanoic acid, my problem is type of solvent to use, because 6-hexaminohexanoic acid is soluble in water but I am not...

02 March 2021 4,443 2 View

Heat exchanger with liquid Nitrogen as coolant?

I want to cool Natural Gas from 195 Kelvin to 138 Klevin in a heat exchanger by using liquid nitrogen as the coolant medium. When entering the heat exchanger, the liquid nitrogen will have a...

01 March 2021 6,450 5 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

For gelatin coating for culturing HUVEC.Should the gelatin be made in sterile water or PBS? Do I need to autoclave the gelatin soln after making it ?

I want to use 0.1% gelatin. Should that be made in PBS or sterile water? Should I autoclave the gelatin soln after making it in water/PBS?

28 February 2021 274 2 View

The reason that the culture of white fungi Contamination with green mold(Trichoderma), the culture autoclave at121 for 15 min. What is it?

Is the period to autoclave not enough? The inoculation in a hood and flame and UV and alcohol.

27 February 2021 9,356 3 View

I want to performe MALDI-TOF MS for bacterial identification, I would appreciate it if anyone can tell me the matrix solution preparation protocol ?

based on what I've studied on multiple papers, for peptide and protein identification Alpha-cyano-4-hydroxycinnamic acid (CHCA) is an appropriate matrix to use, I also know that the CHCA powder...

26 February 2021 6,799 3 View

Why do a weight decrease for thermal degradation in nitrogen gas condition?

Hi all The question is related to TGA(Thermogravimetric analysis). Thermal degradation easily understood but why do samples weight decrease? altough just binding is broked, the weight of...

26 February 2021 1,100 5 View

Steps to calculate activation energy by coats & redfern method?

I'm a chemistry student and I'm researching the kinetic degradation of mixed plastic waste. I'm having trouble calculating the activation energy by the coats & Redfern method. The data that...

24 February 2021 974 1 View

Can I use acetic acid with an ELITE WAX Perkin Elmer column for Gas Chromatography?

Hi! I would want to know if I can use acetic acid with an ELITE WAX Perkin Elmer column for Gas Chromatography. This column has a polyethylene glycol phase, but I can't find the manual to check...

22 February 2021 730 2 View

Can you perform the Murexide test on samples fixed in Formalin?

I have a fixed sample and would like to use the murexide reaction to test for uric acid composition. I no longer have fresh samples

22 February 2021 1,328 1 View