Did my low nitrogen asparagine succinate medium (LN-AS) have gone wrong?

I just prepared solution for LN-AS medium with KH2PO4 (0.2g), MgSO4 x 7 H20 (0.5g), CaCl2 x 2 H20 (0.1g) and succinic acid (1.3g). Everything dissolved completely before autoclaving, but after it...

19 April 2021 9,705 3 View