Did anyone used LED Transilluminator (instead of UV) for visualizing DNA or PCR ... Amplification of bacterial DNA,using 16Sprimer and Midori Green Advance instead of ethidium bromide?
I use the small blue light transilluminator from Clare Chemical. Very happy with it. We don't use ethidium (not allowed it), I have tried sybrsafe, gelred, gelgreen and gelview - all work fine with the blue light. Blue light is fantastic for cloning and allows you to take your time during gel extractions without UV causing DNA damage, giving you more colonies (especially when cloning larger fragments).
LED transilluminator has also UV lamp s no big difference there. We have a good experience here using Visualana dye for our amplicons of 16srDNA visualized in LED transilluminator
I use the small blue light transilluminator from Clare Chemical. Very happy with it. We don't use ethidium (not allowed it), I have tried sybrsafe, gelred, gelgreen and gelview - all work fine with the blue light. Blue light is fantastic for cloning and allows you to take your time during gel extractions without UV causing DNA damage, giving you more colonies (especially when cloning larger fragments).
I bought a LED transilluminator from IO Rodio (http://iorodeo.com/products/midi-led-transilluminator) and saved tons of money. The transilluminator works great and offers lots of advantages over traditional UV/EtBr as described by Nathan.
I didn't get clear bands if using ethidium bromide staining. But the sybersafe works very good with LED and it is safe for environment too.
Thank you all for your help. But I bought LED from dominique dutcher and I used Midori Green Advance instead of ethidium bromide. Unfortunatly I couldnt see any amplified fragment. But wwhen I used UV I saw the fragment. When i asked the company they told me to change it with UV because LED cant read 320 wavelength for DNA.
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