Did anyone try to fix the cells with glutaraldehyde before using ThiolTracker?

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Did anyone try to fix the cells with glutaraldehyde before using ThiolTracker?

I'd like to replace paraformaldehyde in the fixation process, but I am not sure if this is going to work.

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How can I prepare virus for a TEM or SEM imaging?

I have virus (viral hemorrhagic septicemia virus) in suspension and the experiment will not involve cells. What level of TCID50 is preferred?

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Hello researchers Is this a random laser or just fluorescence?

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Could it be a cell culture contamination?

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Is there any way to quantify bacterial and fungal cells in their mixed culture?

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What precautions should be taken while handling S. aureus enterotoxin Type B in the lab?

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Is there anyone with experience in TEM analysis who can assist with a manuscript for an upcoming journal?

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How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

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Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

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Weak DAPI staining after immunohistochemistry - how to improve?

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Lars Krause

Be careful using glutaraldehyde (GA) in combination with fluorescent stains. GA induces more autofluorecence than formaldehyde or paraformaldehyde (PFA). Either you use PFA or a non-aldehyde as a fixative.

Ru-Jeng Teng

I thought ThiolTracker stain live cells so pretreat with fixation may have problem. I have used 4%PFA or 4% formalin before and background fluorescence was a problem if I fix the cells for too long.

Khoa D Tran

GA has autofluorescence issues. You would have to quench it by washing with various buffers. There are a few out there, so pick what you like best. I would suggest getting ultrapure EM-grade formaldehyde (aka paraformaldehyde, usually comes in 16% concentration) and diluting to 4% and fixing with that. Good luck!