16 February 2017 3 8K Report

I used Evagreen as my dye for qPCR. Since I ever tried I always get a slight go down of the amplification plot after the graph reaches its saturation stage. Based on my own understanding, the dye (Evagreen) should be stable binding to the DNA and reflecting the florescence at constant. 

My cDNA template was synthesize by using good integrity of RNA. 

Thank you. 

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