My Xenopus oocytes (healthy frogs) explode before I can make any electrophysiological records, why?

Xenopus laevis oocytes are used in electrophysiology to make transient expression of injected mRNA fragments. Through the technique of 2-electrodes voltage-clamp we can record the physiological...

11 January 2021 6,005 4 View

Transfecting high molecular weight poly I:C using lipofectamine 2000?

I'm planning to transfect HepG2 and Huh7 with poly I:C (high molecular weight poly IC from Invivogen using lipofectamine 2000 as transfectant reagent. I would like to do a standard curve using...

01 January 2021 239 1 View

What is the best washing procedure for Gelatin - arabic gum capsules?

I am preparing microcapsules from complex coacervation procedure to encapsulate an oil phase. However, filtration to remove the aqueous phase results in a paste of aggregated particles. So I am...

25 October 2020 3,152 3 View

Bioinformatic tools to determine ploidy in microbial genomes?

Looking for tools with proved accuracy to detect ploidy levels in microbial whole-genomes.

14 October 2020 4,273 3 View

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26 July 2020 2,893 4 View

Based on your experience, what is the best 3D printing material for teaching human anatomy?

Dear colleagues, We all know that, ideally, human anatomy is better assessed in cadaver model. Artificial 3D-printed moldes, however, emerged to contribute to science. In order to develop...

02 June 2020 7,313 1 View

Synchronous or asynchronous classes during COVID-19 pandemic?

Dear colleagues, what is your approach for remote education during COVID-19 pandemic in terms of timing? What do you prefer? (1) Teaching live with classes pre-established at certain date (e.g....

21 May 2020 6,981 18 View

WRF-CHEM configure start and end of eruption and fall-out?

Hello, I'm using WRF-CHEM to simulate the Etna plume and fall-out. I'm using prep_chem_search.inp to input starting data, using Values.txt tu configure the height, duration, ash mass, and SO2 mass...

19 April 2020 5,503 1 View

Murine alpha-synuclein overexpression in PD mouse model?

I have been reading about A53T aSyn genetic PD mouse model, and I realized that 99% of all of them, are based on the expression of human alpha-synuclein (wild type or mutant) in the mouse. I have...

26 March 2020 4,022 3 View

Improve GPU usage in namd?

I just installed a new RTX 2080 on an old pc (I7 980x 12 CPU cores), downloaded and installed the latest nvidia drivers and CUDA toolkit for ubuntu, also I downloaded the namd build with CUDA...

30 July 2019 375 3 View

Alternative to mediation analysis?

I am currently working on my dissertation and want to explore the role of competence as a mediator between the effect of colour of clothes on voting intention (focus on Western countries)....

21 February 2021 8,609 1 View

Color of spiro-ometad film?

What does the color of a spiro-ometad film indicate? I got purple, green, red, even black color of the spiro film. Is there anything we can get from the preliminary color analysis(e.g. oxidation...

21 February 2021 1,831 4 View

Any information about dinitrosalisylic acid reagent end point color stability ?

I work on estimation of alpha amylase activity using dinitrosalisylic acid reagent If i put the reagent to stope the enzymatic reaction and allow the reagent to react with the produced reducing...

17 February 2021 7,402 4 View

Negative result on natural product sample cholinergic assay?

i have been doing cholinergic assay on natural product and for some of the samples, i get negative value for the inhibition activity. can anyone help me explain the meaning of the negative value...

17 February 2021 4,118 1 View

Has anyone performed a colorimetric RT-LAMP assay using Lucigens LavaLAMP kit?

Hi there, my lab recently opted to use LavaLAMP from Lucigen instead of the Warmstart kit from NEB. However, Lucigen has yet to create a colormetric kit for RT-LAMP, and adding phenol-Red (pH...

17 February 2021 5,399 1 View

What is the actual chemistry going on between iodate and ascorbic acid?

I am studying biochemistry at university, as part of the course we do several titration experiments. In our handouts, we were given the following half equations: IO3- + 6H+ + 4e- I+ +...

16 February 2021 8,731 1 View

Does anyone have a stack of images of action potentials or abnormal brain scans?

I need a stack of black and white .jpeg/.tiff/.jpg/.png images across time of either action potentials, neurons firing, or brain scans (comparing disease and normal brain, disease progression,...

09 February 2021 2,648 3 View

I am looking for a postdoc. Can anyone guide me in this respect?

My PhD thesis entitled as "Anticarcinogenic activity of Terpenoid containing plants against induced Hepatocellular Carcinoma (HCC). My extensive experimental work critically examined the potential...

08 February 2021 8,628 3 View

How can I quantify the color of a solution?

I know colorimetry has been the standard way by way of measuring the concentration of a particular compound (solute) in a colored solution (solvent or measure quantities of a particular compound...

08 February 2021 2,673 1 View

Having 2 different substrates under the same deposit conditions (TiN coating) why it is possible to obtain different colours in the 2 dif substrates?

Coating is made by same deposit conditions. substrates are HSS Drill bit and Shim SS 300 series. On the drill bit we are getting some golden color, however on the shim we got no color it is like...

08 February 2021 9,383 7 View