Ferrozine is heat sensitive compound so it add after autoclaving, the YPD agar medium was autoclaved and cooled to 45oC and then add the Ferrozoine. The Ferrozoine was seperatly sterilized by filtrate sterilization and add asceptically into the agar medium shake well and poured into the plate. this is the best method for the preparation of YPD agar with Ferrozine.