Did anybody experience the problem of getting upregulation of Rcan1-4 expression but inhibition of calcineurin activity?

09 October 2012 1 9K Report

Or in other words, increased nuclear localization of NFAT in cardiomyocytes with no effect or rather inhibitory effect on NFAT-Calcineuring activity detected by luciferase assay?

Is there any other valid and reliable method to measure calcineurin activity apart from NFAT-luc luciferase assay in neonatal rat cardiomyocytes?

Ashraf Rangrez

Thanks a lot Valentina for your informative answer and wishes. I would definitely look into the details of what all things you mentioned here.

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