DiBAC4(3) is an trasmembrane potential dye used for measuring the resting membrane potential. The product description says " The dyes enter depolarized cells where they bind to intracellular proteins or membranes and exhibit enhanced fluorescence."
Here one thing is not clear to me that in a depolarized cell, if the dye enters the cell and binds to intracellular proteins, how can it bind to membranes?
Does anyone also know where it excatly binds in the intracellular region?
Any suggestions are helpful.
Mit
The dye enters the cell membrane but does not go into the cell. It cannot cross the membrane. It stays in the membrane and can bind to membrane proteins.
Also DiBAC4(3) has a rather slow kinetic and is therefore suitable to track slow changes in membrane potential. If you want a dye with faster kinetics then di-4-ANEPPS may be more appropriate.
Take care with these dyes as they are pharmacologic active. For instance they can affect BK channels.
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