Gromacs 2020: how to make distance restrains for 2 atoms in different protein chains?

Sorry, I think it is rather simple problem to solve, but I am stuck on it. I have 2 protein chains, so I have 2 *.itp files for them that are loaded in topol.top. What should I do to use distance...

01 December 2020 1,496 1 View

Different Ct in qPCR with three sets of reference genes primers (each ref.gene is a single copy per genome), with the same template - is it normal?

I am trying to build up 3 standard curves for 3 sets of primers for different reference genes, in order to later use one with the best efficiency. (for SYBR green qPCR, gene copies number...

27 February 2018 8,313 3 View

QPCR with SYBR green - should the amplicons (GOI and reference) be the same length ?

Do I understand right: when running qPCR with SYBR green detection, it is important that two amplicons (GOI and reference gene) should be of the same lenght ? Once It is supposed to be relative...

07 February 2018 4,678 5 View

How do you assess the efficiency/ rate of mutagenesis, when you plate your culture on the selective plate (e.g. canavanine or 5-FOA) ?

After the chemical or UV mutagenesis, the yeast culture is supposed to be plated on the selective medium with canavanine or 5-FOA in order to assess the efficiency of mutagenesis. The questions...

04 January 2018 4,813 3 View

How to do non-water solvent parametrization for Molecular Dynamics?

Currently I need to parametrize some simple F and Cl containing solvents to use them in Gromacs. The problem is that common force fields do not contain atom-types I need, so while understanding...

18 October 2016 7,283 3 View

Could I expect any quantitative difference in EDX results, if the analysis is performed on SEM/TEM samples (same steel and area)?

INCA (Oxford Instruments), X-Max 80 Cr-Ni stainless steel

16 May 2016 7,436 9 View

How to solve the structure of a complex in crystallography?

22 February 2016 2,615 3 View

Could anyone help me with continuous casting modelling in Abaqus ?

11 October 2014 246 1 View

How to avoid fake journal articles published under your authorship without your permission?

01 January 1970 726 4 View

Doping in sport: Questionnaire for sport universities and faculties students and teachers

Dear students and university teachers, This survey is conducted as a part of a study organized by the Prof. Oleg Nemtsev (Department of Sports Disciplines, Institute of Physical Culture and Judo,...

01 January 1970 2,757 4 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Is there any test to determine the cardiovascular risk of patients with obesity? from the perspective of obesity?

We found tests to determine cardiovascular risk taking into account lipid profile, blood pressure, age, history of diabetes, other laboratories. But when weight is involved, there is a paradox. I...

02 March 2021 6,036 1 View

What analysis to choose with a large neste dataset and clearly skewed or kurtotic distributions?

Hello! I have a dataset of n=3000 nested within 8 countries with approximately 200 or 400 responses in each country. I originally planned to perform multilevel modelling with 4 dependent variables...

02 March 2021 6,865 1 View

How to get started building and analyzing a UTAUT model?

Hello all, I need help getting started building and analyzing a UTATU model. I have a survey ready with some questions but I had some confusions on UATAUT and on how to analyze the data. I will...

02 March 2021 9,271 1 View

Do you know any MR image dataset?

I would like to research on MR images (0.5T and 3T). Can you please suggest some websites that I can download dataset including both 0.5T and 3T MR images? Thank you.

02 March 2021 7,735 3 View

Can NFL theorem be valid in infinite search space in ML?

NFL theorem is valid for algorithms training in fixed training set. However, the general characteristic of algorithms in expanded or open dataset has not been proved yet. Could you show your...

01 March 2021 1,189 3 View

Does anyone have kinetic and kinematic dataset for gait classification?

I want to differentiate between normal and abnormal movement. For this reason, I am looking forward to this type of dataset.

01 March 2021 9,485 1 View

Any research paper call for publication related to management or finance or economics?

It should be standard journal (Indexed)

01 March 2021 8,379 1 View

Where can I find public datasets with Covid-19 patient information?

Hi, I'm looking for public datasets that have CT, X-ray information, or medical records for patients with Covid-19. Does anyone have suggestions?

28 February 2021 4,760 3 View

I need dataset for recommandation system?

Hi every body I need to collect datasets from different source to work on recommendation system algorithms, could you advise me how collect data?

27 February 2021 7,063 7 View