I am trying to determine the encapsulation efficiency of liposomes using Triton-X 100 in order to disrupt the membrane followed by extraction of lipophilic drug using hexane..Whenever i put hexane into the liposome suspension containing Triton-X 100, there in formation of milky layer which is producing difficulty in spectroscopic measurement ...Did anyone face similar problem..or any suggestions will be highly appreciated..Thank you in advance..
Try changing hexane with other solvents. The miscibility has to be right and for that check the miscibility first with the Triton-X and other solvents.
If your drug is soluble in hexan , you can centrifuge it to get a transparent sloution then detect the amount of your drug in it
Dear Ajit Kumar Pratihast ,
In reply to your question:
"I am trying to determine the encapsulation efficiency of liposomes using Triton-X 100 in order to disrupt the membrane followed by extraction of lipophilic drug using hexane ..."
First of all you should specify what equation you are using to calculate the EE of your formulation? Most researchers evaluate EE by considering non-encapsulated material and this is acceptable by the scientific community (including peer-reviewed journals) in the field. Hence you do not need to disrupt the liposomes in order to extract the encapsulated / entrapped material.
Disruption of liposomal bilayer is not a simple task to achieve easily and might affect the composition of the phases you separate after separation (e.g. by centrifugation or filtration) and will make quantification of the active compounds complicated.
On the other hand, Triton X-100 is a non-ionic surfactant and emulsifier (see attached picture). While other non-ionic surfactants (e.g. Tweens, Spans, etc.) are being used to manufacture niosomes, the expected effect of Triton X-100 on liposomes is disruption of the phospholipid bilayers (depending on the ingredients and composition of the bilayer). In some (if not many) cases in practice this does not happen.
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