Distinguishing between human activated and exhausted lymphocytes T?

Any suggestions on how to distinguish between activated and exhausted human lymphocytes T CD8 with flow cytometry? It is known that exhausted lymphocytes T have higher expression of inhibitory...

28 December 2020 255 3 View

Is this conference a scum?

Hi, can anyone help me to understand if this conference about phytoremediation is a scum? https://waset.org/phytoremediation-phytotechnologies-and-management-conference-in-july-2020-in-prague...

15 January 2020 8,841 3 View

Help with lentiviral vector?

Hi everyone, I am trying to produce eGFP lentiviral vector by transducting HEK 293T with calcium phosphate method. I transduce cells when they are at 70 % confluent (following this method...

14 October 2018 2,351 4 View

Cross-sex genetic correlation

Hi! We are trying to estimate body mass (W) heritability and cross-sex genetic correlation using MCMCglmm. Our data matrix consists of three columns: ID, sex, and W. Body mass data is NOT...

25 July 2017 6,702 1 View

Which are the best melanoma cell lines?

Hello, I'm working on melanoma and I'm searching the best melanoma cell lines (easy to culture) recapitulating all the steps of melanoma growth: Radial growth-phase cell line, vertical...

05 June 2017 8,061 2 View

I would like to extracte cell membrane from frozen buffy coat. Can anyone suggest a reliable protocol?

Hello everyone, I need to isolate cell membrane of leukocytes and platelet, from frozen buffy coat. Any suggestion?

20 September 2016 5,749 1 View

Fibronectin antibody clone FBN11?

I wish to stain fibronectin in 3D tumor spheroids made with human cancer cells and fibroblasts. do you have a good antibody to suggest? Have you ever used the references MA5-11981 from...

30 June 2016 1,973 2 View

Does the rule of choosing a component if the model performance increases by >5% measured by RMSEP (in cross-validation) apply also to PLS technique?

When choosing a WAPLS model, apart from relying on performance (e.g. r2, r, max bias, RMSEP, scatter-plot etc..), the recommendation is to select the successive component if the model performance...

15 May 2016 1,200 2 View

How can I concentrate down some digested liquid coming from in vitro digestion without disrupting its micelles?

20 April 2016 3,381 3 View

How can I effectively debenzylate a tertiary amine?

31 March 2016 9,050 8 View

What is your preference for journals for publishing in the field of Hyperspectral Imaging or Remote sensing?

I believe that we need to share our experience in publishing in the field of Hyperspectral Imaging or remote sensing? What is the best journal that you publish with? What is the worst experience...

23 February 2021 3,411 4 View

How does trust influence the development of environmental projects?

Hello Scholars, I am exploring "trust" relevance in the development of socio-technical systems. What is your perception of cognitive values like trust to the energy transition/implementation of...

09 February 2021 8,133 5 View

Cross-country research: How can I control for the factor country, although I don't have any hypotheses regarding country differences?

Hello, I am currently preparing a preregistered report for experiments in different countries. I am studying motivations/values of students; therefore, I want to conduct several same experiments...

28 January 2021 5,763 3 View

Does anybody know about literature on protest and post-conflict processes/societies? What would you recommend?

I am trying to start a project on the subject and your help would be appreciated

18 January 2021 2,190 6 View

How to adjust a covariate in meta-analysis?

Suppose I am meta-analyzing proportions using their logit transformations. We did a meta-regression of the covariates age, gender, and disease severity score in studies and get respective beta...

11 January 2021 9,409 4 View

Are international conferences a dying species?

In our discipline (chemistry), annual meetings of the American Chemical Society (ACS) used to be mega-events with up to 20,000 participants. The current one will be virtual, and the organizers...

06 January 2021 1,882 5 View

Could you name a few country (developing) worst affected due to corruption?

The impact of corruption in education is unbearable, as witnessed out from the sufferings of society in all different aspects of life. Can you please provide a comparative impact on the percentile...

03 January 2021 2,725 8 View

Homeopathy is within the reach of most of the financial class?

Greetings. I personally have taken homeopathy medications and found it effective in curing. I do not know if it is effective in all kinds of diseases, but it is within the financial reach of all...

02 January 2021 9,904 5 View

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01 January 2021 5,831 1 View

Writing participant characteristics in method while being LGBTQI+ sensitive?

I apologize in advance if I come across as ignorant. I am trying my best to educate myself on LGBTQI+ community and how to write them in academic writing. I have a sample of X participants in a...

29 December 2020 9,011 3 View