I have tried to detect Plasmodium falciparum lactate dehydrogenase directly from whole malaria infected blood using electrochemical impedance spectroscopy without success. I made dilution of the blood with buffer, PBS, and noninfected blood all to no avail. I wish anyone on this platform could be of help with an idea to solve the problem. Thank you
Dear Adewoyin,
Looks like you have a problem with concentration/diffusion of PfLDH. Have you ever tried a rotating disk electrode? The impedance spectra changes a lot, but I think it could be a solution for it.
Another solution could be using a electrochemical quartz crystal microbalance due to the very small quantity you want to measure.
Hope it helps.
Thank you. Your first response solved the problem. The concentration of the PfLDH was too high. As soon as made dilution to about 1000 fold the aptamer was able to bind with a corresponding high charge resistance.
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