Hi ladies and gents. I'm trying to detect a fluorphore-conjugated antibody linked to a solid surface using a synergy plate reader. My question is: would I be better off running the analysis in wells with or without a buffer solution?

I am concerned on the one hand that the buffer would quench the emission from the object in the bottom of the well. On the other hand, I don't know if the antibody/fluorophore being dry will denature it to the point that it will no longer fluoresce.

Any ideas?

More William Francis Burke's questions See All
Similar questions and discussions