I recommend you to use 10% CO2, 37 Celsius, pH=7 and 100 rpm. Culture of Helicobacter pylori using these parameters gives good results.

Thank you Pawel,

which broth medium you use ?

hii Pawel

you have a good article about this, i see that you work on Helicobacter pylori projects?

Normally, I use BHI liquid media with 7% fetal bovine serum. Example:

Article Streptococcus mitis Induces Conversion of Helicobacter pylor...

Other authors also suggest the usefulness of other culture media or supplements (e.g. β-cyclodextrin, charcoal, or starch):

Article Perspectives on Methodology for In Vitro Culture of Helicoba...

Article Liquid culture medium for the rapid cultivation of Helicobac...

Hi, Ammar I recommend homigening the gastric tissue

under sterile conditions in 200 μL of saline solution 0.89% using a homogenizer (Kimble-Kontes, Vinelan, NJ, United States). The homogenized was placed on Columbia agar plates (Oxoid, Basingstoke, Hampshire, England) with defibrinated sheep blood at 7% plus selective supplement for H. pylori (Dent) containing Vancomycin (10 mg/L), Sodium cefsulodin (5 mg/L), Trimethoprim lactate (5 mg/L), and Anphotericin B (5 mg/L) (Oxoid, Basingstoke, Hampshire, England). The agar plates were incubated under microaerophilic conditions (6% O2, 6% CO2, 88% N2, using CampyPak Plus envelope, BBL, Nashville, TN, United States) at 37 ℃ from

four to eight days until observing small gray translucent colonies. Good luck.