I am investigating on isolation and identification of cry genes in Bacillus thuringiensis.
After isolation Bacteria according to the related papers, gram staining was conducted. Also, commassie blue used and the bacteria were checked for crystals. Then, a bioassay was performed on Ephestia kuehniella and the results showed that several bacteria caused mortality on the floar moth even more than Bt subsp thuringiensis. But, for PCR by primers, no gene was detected. What is the reason?
M. Rashki
If your bacterium has crystals that you can observe by means of phase contrast microscopy, I recommend that you do an exploratory search with primers designed from block 1 and 5 of the cry genes, so you have an idea of the genes that you can have your bacterium and from that design specific primers for the gene that codes for your protein.
If you like more information or something in particular you can contact me.
Regards,
J. Francisco Castillo E.
Dear José Francisco Castillo-Esparza
Thank you for your answer. Could you please send me your email?
Yours,
Maryam Rashki
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