Someone help me thanks a lot
Have you ever used dilution and plating methods for this? These methods are much useful in culturing soil
Dear all, I am searching about how to conducting SEM analysis. Could you please clarify the difference and conditions to use for each model?
05 June 2020 6,496 4 View
In practical machining by EDM. we always install dimensions of discharge openings on the machine in order to ensure the accuracy of processing.How can measure dimensions of discharge gap in EDM?
25 March 2016 7,134 4 View
Please tell us: electrode wear affects how the quality of EDM machining?
20 February 2016 3,249 4 View
After I cryofreeze the cell do I need to remove the DMSO for culturing the cell for next passages .
28 February 2021 7,738 3 View
I want to use 0.1% gelatin. Should that be made in PBS or sterile water? Should I autoclave the gelatin soln after making it in water/PBS?
28 February 2021 274 2 View
Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...
27 February 2021 3,904 2 View
I've been having a hard time forming an endothelial monolayer with good tight junctions. The person who trained me on the culture of endothelial cells worked mostly with mouse BMEC, and he used...
22 February 2021 8,253 1 View
Hello. I want to isotopically label the my protein, and I express it in BL21 RIPL using M9 minimal media. It grows extremely slow. I inoculate the colony from a fresh M9 plate into 5ml M9 medium...
17 February 2021 2,852 2 View
I need to assimilate a determining condition for the plant. However, I need to ask you if it is possible to change the properties and concentration of plant nutrients in this material like perlite...
04 February 2021 8,616 11 View
Hi there! My lab has been brought an array of C-4 I cell line. The cells did not succeed in attaching to the bottom of the flask and remained floating in the media. Although we followed the...
04 February 2021 10,018 5 View
Hello there! My lab has recently been (very graciously) gifted an array of cell lines, of which include 67NR and 4T07 cell lines. While I've found a decent amount of background and culturing...
02 February 2021 2,590 3 View
Hi everyone, I have been culturing HEK293T cells that have been engineered to overexpress the mechanosensitive protein Piezo1. However, I am finding it very difficult to keep them in culture -...
02 February 2021 7,892 4 View
Hi all, How would you use qPCR to quantify the amount of a particular bacterial species in a sample experimentally? I understand you could use specific primers for the 16s V region of a...
01 February 2021 7,153 4 View