How to know if Zinc Oxide is coated on the glass substrates?

Hello all I have prepared Zinc Oxide nanoparticles using sol gel synthesis. I tried to coat the ITO and glass substrates with the ZnO nanoparticles ( dispersed in ethanol). But I am not sure if...

08 December 2020 3,692 8 View

Are you equipped to count small plasmid chromosomes?

I wish to perform an experiment which will provide support for a novel DNA structure. Most of the experiment is conceptually simple, easy to perform, and inexpensive. But the key to doing it is...

29 November 2020 5,258 9 View

Which PEIE to use?

Hello all Thanks for answering my previous questions. I am making perovskite LED and intend to use PEIE on zinc oxide layer as ETL. But I am not sure what version of PEIE to us. There is the one...

08 November 2020 5,710 3 View

Zno NPs seperation?

Thanks a lot for your answers. I have one more question: after synthesizing ZnO nanoparticles , I am not able to separate from the original solution (dispersion actually). The recipe is as...

23 October 2020 9,857 8 View

How to do PBABr synthesis?

I am following this recipe for PBABr synthesis. I added HBr (48 wt % in H2O) to Methanol and PBA solution at 0 degrees celsius. But after 2 hours of stirring I am not able to see precipitates. I...

08 October 2020 6,485 4 View

ZnO and PBABr synthesis?

Hello all, I want to synthesize ZnO nanoparticles and PBABr in my lab for my LED project. I know the method, but I am not sure how can I check(even visually) that I have arrived at the correct...

27 September 2020 5,334 7 View

MoO3 RF Sputtering?

Hello all, I have to do RF sputtering for MoO3 for my LED project. But I have come across many recipes for reactive RF sputtering of MoO3 ,using Mo as a target and Oxygen as the reactive gas But...

16 September 2020 5,222 2 View

Can we define the external quantum efficiency as the (number of photons emitted/ electrons injected) for n LED device?

I have come across the definition for EQE as the number of radiative recombination events out of the total number of recombination events in the LED. But is it safe to say that EQE = number of...

07 July 2020 3,023 1 View

How to obtain corrected PL spectrum?

Hello all, I am intending to measure the PL spectra for my perovskite samples. How can we obtain a corrected PL spectra in the spectrometer, independent of the photoresponsivity of the silicon...

29 June 2020 7,298 5 View

How to plot gaussian distribution on Matlab?

15 May 2020 9,150 3 View

How to separate water from glycerol solvent in a solvent recovery vacuum distillation column?

I am currently doing my third year design project. I have been assigned to design a glycerol recovery column in vinyl acetate monomer production. The process unit before the recovery column is an...

01 March 2021 9,525 4 View

POlymer+DMSO solution and moisture??

What could be the reason of moisture at the sides of Vial when I am preparing my polmyer+DMSO solution for membrane fabrication? What etiquettes I am possibly missing here?

28 February 2021 6,516 5 View

Can I use TCA instead of TFA for protein analyis in RP-HPLC?

I am trying to analyze lipase using the reverse phase-HPLC C18 column. In the literature, 0.1 % TFA was and 60% ACN was used. Can I use TCA in place of TFA? If any other methods are available to...

25 February 2021 2,388 3 View

Multi effect distillation modeling in EES?

Dear colleagues If anyone has a model of multi-effect distillation in EES or MATLAB software, please send it to me.

24 February 2021 7,282 1 View

IF troubleshooting: looking at an intracellular protein that localizes to the cell's and how to reduce increase in signal due to cell ruffles?

I am currently trying to stain for PI3KBeta in MDA-MB-231 cells. My current issue is that 1) beta stains all over the cell and 2) there is an increase in signal at the edge of the cell and this...

24 February 2021 8,582 1 View

Cloudy/high background western blot - can anyone help me?

Hi everyone! I try to detect VDR protein (~48 kDa) using Western Blot. After SDS-PAGE using 10% MP TGX Stain-Free Gel from BioRad I transferred the protein onto an metOH activated PVDF membrane...

23 February 2021 6,266 3 View

What is the best etching method for Mg-Ca alloy?

I have used Picral etchant (4.2 g Picric acid, 10 mL Acetic acid, 70 mL Ethanol, 10 mL distilled water) but it didn't work. Is there any alternative?

21 February 2021 2,558 5 View

How to dilute Toxin-DMSO solution?

I have a concentrated solution of a toxin(50 micromolar) in DMSO 100%. I need to dilute it inorder to dilute both the toxin and DMSO wich can be toxic to cells. Which would you guys recommend to...

19 February 2021 7,447 2 View

How to improve the buffer capacity of sea water?

Hello everyone I would like to improve the buffer capacity of seawater (pH ~8) in order to dissolve as much CO2 as possible without dropping the pH below 7.5 (a range between 7 - 7.5 is...

17 February 2021 2,797 2 View

What are the best cations for preparing competent cells, Ca2+ or Mg2+?

I have been reading various articles and most state that treatment with CaCl2 produces higher efficiency of transformation than MgCl2. Can anyone explain why Ca2+ is better than Mg2+ at...

16 February 2021 8,519 5 View