How many tumor cells should I seed in a 96-well plate/well for virus infectivity?

The tumor cells are from a variety of cell lines from ATCC .

22 December 2020 1,950 3 View

How does band gap of a semiconductor shift with the lattice strain? and physical significance?

Someone posted a similar question but I have a further question on strain (increase/ decrease atom distance). So I know that the distance increases and decreases the bandgap distance. But can it...

04 June 2020 689 5 View

Goodmorning..when doing genetic analysis of grain yield and yield attributing traits for drought tolerance and yield attributing traits self pollinat?

Goodmorning..when doing genetic analysis of grain yield and yield attributing traits for drought tolerance in self pollinating crops (F2 generation) Vs cross pollinating crops, is it important to...

30 March 2020 1,011 3 View

What is the most appropriate statistical test for my experiment?

I am having trouble identifying the most appropriate statistical test for an experiment I am designing. In this experiment I am looking at multiple markers that indicate a change in structure...

25 November 2019 9,090 2 View

Does anyone have any recommendations for good, clean antibodies they have used for IHC in the mouse lung?

I am new to the immunohistochemistry world and am currently about to teach myself the basics on some sections of C57BL/6 mouse lung. Does anyone have any good antibody recommendations or proteins...

28 October 2019 602 3 View

Where could I find some SOPs and formulations for making in house Clinical chemistry reagents?

Hi, I am looking for some resources to be able to find some SOPs and formulations for making in house Clinical enzymatic chemistry reagents like; Triglyceride, HDL-Cholestrol, Oxalate, Lactate,...

25 July 2019 698 2 View

Has anyone else had any problems with PSA antibodies for WB?

I am doing work on prostate cancer cell line - LNCaP - and using PSA antibody to show androgen deprivation. We currently have 3 in our lab, none of which are giving us a signal for western blots....

14 July 2019 2,723 3 View

How to adjust GEE analysis for the pre values on SPSS?

I have a 2 (group) x 3 (time) analysis performed and there is a difference between the pre values and I'd like to adjust it (using the pre values as covariates). However, on SPSS since I need all...

31 March 2019 7,088 5 View

When thawing cryopreserved cells, can they be added to 0.9% NaCl without loss of viability?

The cells are cryopreserved in 8% DMSO and 2% HSA.

08 November 2018 3,807 3 View

What is best way to check for non-proliferation in a tumor cell lysate?

We use 6x freeze-thaw cycles and consistently show 0% viability by Trypan Blue. We have used MTT and results were good, but some say DNA assays are better. Thanks

03 August 2018 4,119 3 View

Differences between sheep blood with Alsever solution vs citrated/defibrinated sheep blood in microbiological media?

I am aware of a study that has compared the use of citrated and defibrinated sheep's blood in microbiological media (Hair Sheep Blood, Citrated or Defibrinated, Fulfills All Req... ), but can...

10 February 2021 5,927 1 View

Can anybody provide me sequences of SPF10 primers?

03 February 2021 1,053 2 View

How do you annotate figures to show significance?

30 January 2021 1,008 3 View

Can Vibrio campbellii bioluminescence be obtained when cultured on Mueller-Hinton agar?

Need to check quorum sensing activity of Vibrio campbellii.

20 January 2021 570 5 View

Can anyone suggest a commercial MAT kit to detect Toxoplasma gondii antibodies in animals?

18 January 2021 5,438 2 View

Can anyone help for the content validity of my psychological test?

Good day! I am in the midst of completing my test development requirement under the Psychological Assessment subject. I was wondering if anyone can help me reach experts or psychology graduates...

14 January 2021 1,536 4 View

Which neuropsychological tests assess immediate and delay(24-48hrs) declarative memory/learning (verbal and visual) and procedural memory in children?

it can be either a neuropsychological assessmentfrom age 8 years old or a wel research paradigm

13 January 2021 2,308 2 View

Spatial Panel Diagnostic Test?

Hello Colleagues Trust you are all fine, Please I have been struggling for days on estimating some spatial diagnostic test on my model. The stata command I am using is spatdiag command. I am...

04 January 2021 3,919 1 View

Whether new variant will affect the diagnostic method of detection or not?

can it be diagnosied by the same tool or different tool

04 January 2021 2,964 2 View

Has anybody knows about Anti-COVID-19 antibody- anti-Viral COVID-19 Nucleocapsid (NP) Humanized Coronavirus?

In order to establish new Rapid Diagnostic Test, I need the mentioned Anti-COVID-19 antibody urgently. Has anybody can assist me to prepare it? Thanks in anticipated

31 December 2020 635 5 View