Hello everyone, i´m just introduce myself in Minion sequencing and i need to run 1200pb amplicon. How can i calculate how many samples load in the minion, i know that´s depends of my study and i guess that 1000x of depth is enough.
Hello, I am currently analyzing some phosphoproteomics data, but I have peptides with multiple phosphorylation sites or phosphorylations together with carbamidomethylation or oxidation. How can I...
04 August 2024 8,432 3 View
A typical reforming section in a large-scale Haber-Bosch process (Figure 1) comprises two reactors. A mixture of methane (CH4) and steam (H2O) is fed into a primary reformer to produce syngas via...
01 August 2024 7,051 5 View
Hello, We are designing a protocol to determine the effect of certain compounds in the health of rats, but our problems have certain instability issues when subjected to certain pHs. We were...
19 June 2024 5,950 1 View
Hello to everyone, I'm trying to extrtact total RNA from sEVs. I made a first experiment starting from 2x10^10 sEVs quantified by NTA but the RNA obtained was very low (under 20ng/ml) Do you have...
18 June 2024 2,799 4 View
I am working with electroluminescence (EL) imaging of photovoltaic panels and need to ensure that the modules are not damaged during testing. I understand that the bias current is usually set...
16 June 2024 5,514 1 View
Hi everyone, I need help and yuoue experience!!! What kind of cell culture contamination is it? Video attached: The cells are lung cancer cells that are thawed from -80°C fridge and we known that...
04 June 2024 9,619 2 View
Hello, these samples comes from Olinalá Formation (Permian age), Guerrero State, México. I need help to know if they correspond to Roadoceras, Stacheoceras and Waagonoceras (that have been...
27 May 2024 9,110 0 View
Electrochemistry is a pathway to convert CO2 into valuable products such as CO. However, a potential side product in the cathode is H2. I wonder if it is possible to tune the composition of the...
12 May 2024 9,061 3 View
I am trying to establish a model of hypoxia-reoxygenation in HK-2 cells in order to determine their expression of kidney injury biomarkers (e.g. KIM-1, NGAL, etc.) via qPCR. However, I am finding...
09 May 2024 9,223 0 View
contextualiza LA ADECUADA gestion residuos solidos nivel mundial y local con formato APA 7 para un marco teorico de tesis doctoral
05 May 2024 3,257 0 View
Dear QE-users, In the method where full MS positive mode and PRM mode are used, we always get an incorrect auxiliary gas reading (41 instead of 25). This only happens in this method; other...
06 August 2024 4,953 0 View
Hello guys! Do you have experience running a Oaxaca-Blinder decomposition on R applying person weights. How do you suggest doing it? I have a variable PERWT which gives more information on how...
04 August 2024 6,033 0 View
It's an end-point PCR protocol. I'm using 1.5% agarose gel with SyBR Safe dye and TBE as a running buffer, visualization on BioRad XR+ system. I was primarily thinking of primer efficiency,...
01 August 2024 4,673 4 View
Hello Everyone I have a question about structure for connectivity analysis on sources. My goal: preprocess and cut data into trials create headmodels, using template MRI file perform source...
30 July 2024 2,744 1 View
Hello all, I have been trying to follow a 2-stage PCR protocol used to amplify barcodes of a large yeast library, as per Nyugen et al. (2022) -...
30 July 2024 841 2 View
When I run autogrid4 it says: autogrid4: ERROR: Unknown receptor type: "Cr" -- Add parameters for it to the parameter library first! Look forward to your reply.Thank you so much!
29 July 2024 488 0 View
I am trying to run a restart analysis, which imports deformed configurations of parts from a generated ODB file. It runs fine in Windows OS but when I try to run it in Linux OS, it is giving some...
29 July 2024 9,572 3 View
Our department has recently acquired an HPC (High-Performance Computing) system, and I'm thrilled to take my molecular dynamics calculations to the next level using Desmond. I used to run my...
28 July 2024 6,553 1 View
I am preparing to run a lot of samples on a 384 well plate and I wanted to test if any well in my PCR machine is dead so as not to lose any data. I would really appreciate help with how I can do...
24 July 2024 8,172 1 View
Hello everyone, I am conducting
22 July 2024 8,085 11 View