How to handle milk clotting Issues during media preparation for Screening of Halotolerant Protease Producing Bacteria (with Skim Milk)?

We want to screen halotolerant protease producing bacteria. Since Skim milk (HiMedia) is available in our lab, therefore, we want to screen extracellular protease by this compound/ supplement. We...

04 December 2020 7,657 6 View

What are the mechanism of actions of Ag and Cu nanoparticles on the bacteria and fungi?

We are assessing the activity of nanoparticle incorporated fabrics against bacteria and fungi.

24 November 2020 8,686 6 View

How to estimate nitrogen content (%) in plant using pelican equipment's KELPLUS ELITE-EX VA semi automatic kjeldahl distillation unit? ?

I am using KELPLUS ELITE-EX VA semi-automatic Kjeldahl distillation unit for nitrogen estimation. The salesman told me that unlike the older version; this version does not require making up the...

01 October 2020 844 3 View

Why Magnesium Sulfate is used in Loop-Mediated Isothermal Amplification(LAMP) reaction instead of MgCl2?

We have designed an in house LAMP assay for the detection of a pathogen. We have used ThermoPol buffer, betaine, MgSO4, Bst polymerase and dNTPs in the reaction. I wanted to know why MgSO4 is used...

22 September 2020 6,916 5 View

Cloning in TRV2 vector: tips?

So, I have been cloning a lot of genes over the years in various plant transformation and overexpression vectors and even though its a struggle always to generate positive clones, I usually am...

26 August 2020 8,364 9 View

Is it possible to hire a portable photosynthesis system in Northern India?

I have an experiment in which I want to estimate the photosynthetic activity and carbon dioxide flux of wheat plant. However, in my institute does not have potable photosynthesis equipment. Is...

11 August 2020 9,701 2 View

Is it possible to publish research paper on plant nutrition having only single year of research work in a Thompson and Reuters IF Journal?

As in Masters of Science in Agronomy in many places have only one year of research work, is it possible to convert it to a paper and publish it in an impact factored journal especially when the...

04 August 2020 4,252 6 View

Has anyone used CABS flex 2.0 for molecular dynamics simulation of docked protein-protein structures?

Hi all, I need an info on the CABS flex and how it works for protein protein interaction simulations. I have docked structures obtained using cluspro and would like to subject them to MD. Please...

24 April 2020 1,509 1 View

What is the difference between Bubble type and Vapor type Precursor?

I am working with La(PrCp)3 Precursor in my Lab. I am not getting enough thickness after oxide layer deposition with ALD. To solve this issue I want to know about the precursor's chemical...

16 February 2020 5,640 3 View

How to register an indigenous variety in India?

The farmers are cultivating an unregistered land-race for a long period; if I wish to register this variety, what are the legal procedure and how to register it.

14 February 2020 525 3 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Discrete Phase model (DPM) particle break-up has particle size limits during particle tracking?

Hello, I am currently working on simulating water droplet partciles in a closed room. When I use uniform particles, without "particle break-up", I can carry out the simulation without any problem....

02 March 2021 3,053 2 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

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01 March 2021 3,905 5 View

What test should I use to compare two or three means, if data is not normally distributed but sample size is > 30 in each group?

I personally feel this as a most confusing question. As per my understanding, if sample size is > 30, and sample is skewed, we can still use paramteric approach, as it will follow Z...

01 March 2021 1,587 6 View

Is it necessary to report effect size (ES) in statistics of biomedical research given that we already report p values routinely?

Pls share your thoughts

01 March 2021 7,330 4 View

Distribution of particles in metal matrix?

How would define the "good" and "poor" distribution of particles within the matrix. Is there a quantitative solution to assess the distribution of the particles, which then having denotation of...

28 February 2021 7,397 8 View

Is there a deep reason for using the squared modulus of the wave function as the probability density of the existence of the particle?

I think we were all surprised at the first time we got to know quantum mechanics that the squared modulus of the wave function is the probability density of the existence of the particle? The role...

28 February 2021 6,282 9 View

Zno nano particles colour changed from white to grey in process of calcination any suggestions?

In the formation of Zno nano particles process, after drying at 120 degree C, my zno nano particles are completely white but when i calcine it for 450 degree C it converts into grey colour. i...

26 February 2021 2,435 4 View

Why there are two peaks while using the mie plot of Silver nanoparticle?

Hi, I am trying to learn the theoretical modelling of silver nanoparticle using the Mie scattering theory. After that, I discovered a software named Mieplot developed by Philip Laven, where it...

25 February 2021 7,493 6 View