If anyone already have experienced in this protocol please specify and suggested to me. I already quantify total protein content (Lowry method). So i need only quantify the keratin content.
Dear Rama, a great question for which I too have been looking for an answer for quite some time. Most of the assays in this are epidermal assays in mammals. Question is which keratin are you looking at? on which organism?
I have published a few papers on ptilochronology which indirectly quantifies through growth bars the relative keratin density. But would like to know how we could assay the relative quantity of keratin in each of the sections of the feathers.