Dear biotechnologists, i am going to do a double digestion for a plasmid that contains the insert inside (GOI), by EcoRI and HindIII?

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Mohammed Al-Nussairawi

Guillermo Posadas-Herrera

Dear Herrera,

I have attached the photo from the thermofisher ... yes HindIII should be doubled in size ( if EcoRI 1 microL , HindIII means 2 microL IF I used 2x tango)

Many thanks for your answer

Maria Evers

Check out snapgene software, it has a very convenient feature for restriction enzyme digestions (buffers, temps, etc.)!