How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

How to analyse data without control group in meta-analysis?

I am interested in knowing the treatment effect in my study. I have data (mean, SD) for pre and post treatment.

01 March 2020 3,572 2 View

The pH differential method - how do i calculate the concentration of anthocyanin in a dried extract?

27 February 2020 9,573 5 View

How to begin slicing sections after a period in cryoperservation?

I have preserved large sections of sheep brain tissue in an ethylene glycol solution for later use. I'm hoping to take the sections out of solution and then slice on a microtome or cyrostat. How...

29 January 2020 1,884 3 View

Please i need an expert help on how to convert amino acids results expressed mg/l to residues??

Please what formula should I use to convert amino acid compsition results expressed in mg/l to residues? I ran a HPLC amino acid profile of a protein sample, the results came in mg/l but I need to...

18 January 2020 5,765 1 View

Is anyone aware of a study that compares the discourse around intra-European mobility and integration for Eastern and Western Europeans?

I am looking for a study that compares the discourse around intra-European mobility and integration for Eastern and Western Europeans. Mobility of Western Europeans is not addressed in public...

20 August 2019 3,036 2 View

How does one calculate the % metal extraction, distribution ratio for each pH value, log D and pH1/2 from log D vs. pH plot from AAS results?

21 May 2019 8,115 3 View

In how many days maximum should I change the medium (L15) of breast cancer cells ?

01 February 2019 9,569 2 View

Does anyone know of some working antibodies against EEA1?

I am looking for a good antibody to label early endosome of mouse cortical neuron.

18 November 2018 3,875 2 View

Have you used Covidence for systematic reviews before?

I came across Covidence for systematic reviews, which is the tool of choice for Cochrane, and was wondering if actual users can share their experience with the tool. It looks like a pretty...

06 August 2018 8,846 11 View

How to find the mean, SD and CI in SPSS?

Hi, I hope that someone can help me with this task: I have 5 groups of participants which are numbered in SPSS from 1 to 5 (according to their group number) and are all under the variable group....

03 March 2021 506 3 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

Can you share me how teacher education is managed in your respective national educational system?

I feel that the practice in teacher education in my country is below the expected performance level due to very poor management system. Hope I will learn something from your experiences.

02 March 2021 1,516 4 View

Which SAR dataset to use for generating coherency matrix and performing target decomposition (Description) ?

Working on chandrayaan-2 DFSAR data, there are three datasets available: 1) Slant range image data product: The slant range complex image file. Each pixel is represented by two 4-byte floating...

02 March 2021 8,481 3 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

How to simultaneously perform multiple biochemical assays?

I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...

01 March 2021 6,999 3 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

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Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View

MiRNA qpcr problem?

Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.

28 February 2021 5,008 4 View

Runs of nucleotides in Sanger sequencing?

I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...

27 February 2021 547 3 View