How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

No title

03 March 2021 5,360 2 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

The procedure compute?

02 March 2021 5,204 3 View

What is corrct way to define ports when simulating FSS and surface waves in CST?

I need to simulate a mushroom FSS and I'm struggling with the ports. What's the correct way to define them? Should I construct a proper SMA connectors, or is it enough to put a waveguide ports...

19 January 2021 6,590 4 View

Can anyone identify this fungus/mushroom from China?

The pictures were taken in Guishan Mts in Yunnan. The fungus is ca. 10-12 cm high.

07 January 2021 6,639 10 View

Hi Is there any one, who help me in processing my raw data files of some mushrooms of LC-MS , by using MZmine2, MS- Dial etc ?

I have received some of my LC-MS raw files but I can not process it by using MZmine2. MS Dial, for compounds detection. Thanks in advance.

02 November 2020 3,564 5 View

No title

mushrooms play a vital role in human life used for food, medicine, and chemical industries, many edible mushroom-like morels can not be grown by a human, and this type of mushrooms are called...

18 October 2020 1,457 12 View

What are the equation and conversion factor to estimate protein content from mushroom?

Total nitrogen was calculated with Kjeldahl method, I found different equations and factors ''Total nitrogen content of shrimp waste was determined using the Kjeldahl method. Separately, pure...

17 August 2020 4,572 9 View

Does it matter what order of extraction solvent is used for extracting bioactive compounds from medicinal mushrooms (elaborate description below)?

Hi, My question is regarding medicinal mushroom extraction, but may also apply to broader extraction knowledge of all biota. I understand, that water and ethanol as extraction solvents (along...

25 June 2020 1,113 9 View

How to work with diversity indexes data while working with aleatory transects inside an area?

Hello! I've used the aleatory transect methodology to survey for mushrooms in six different areas. We've done 4 samples (transects) in which area along 4 months , one per month. I've already...

07 April 2020 9,664 3 View

Why we got more than one repeat for each primer used in NGS applied for DNA sequencing from each sample of date palm tree ?

Our project objective is to investigate the genetic diversity of Palestinian date palm, cultivars. 6 primers were ordered, these primers were designed specifically for NGS analysis. DNA was...

06 February 2020 695 2 View

Why we got more than one repeat for each primer used in NGS applied for DNA sequencing from each sample of date palm tree ?

Our project objective is to investigate the genetic diversity of Palestinian date palm, cultivars. 6 primers were ordered, these primers were designed specifically for NGS analysis. DNA was...

01 February 2020 2,733 1 View

How we can determined the composition of oil palm trunk sap?

How we can determined the composition of oil palm trunk sap? I mean what type of ingredients are present in sap and what are the ingredient percentage in oil palm trunk sap

01 February 2020 5,352 5 View