Can DMSO be a positive control in a necroptosis assay?

Dear all, I would like to ask, if DMSO can be a positive control in this particular experience. L929 murine cells stimulated by murine-TNF-Alpha by 8 hours mainly leads to cell death by...

21 June 2018 2,136 2 View

Flow Cytometry protocol for Tregs detection (CD4+, CD25high, FoxP3+) in fresh isolated horses PBMCs ?

Hello, I'm Trying to mark Tregs population in Horses, I adapted a protocol used to mark Tregs in dogs, and It worked at the beginning. But after 7 months I started my experiment and I haven't...

25 May 2018 7,953 5 View

Looking for a Toloudine blue protocol for skeletochonology analyses?

Hi, I am looking for a Toloudine blue protocol for skeletochronology analyses. I will use lizard femurs, bones will be embedded in paraffin. Specimens were injected with 10% formalin, and are...

04 May 2018 6,746 0 View

Do you usually submit presubmission enquiries? What are the advantages and what do you usually write to get the best feedback?

Do you think it is something worth doing?

28 April 2018 7,654 7 View

Western Blot bands change after second antibody exposure.

Dear all, I have an interesting problem. I performed a WB but the initial primary antibody incubation produced weak bands after 20 minute exposure. So I reincubated with the same antibody solution...

14 May 2017 9,484 2 View

Can anyone suggest research papers on Social Science gamification?

I need some papers about Gamification but in Social Science

06 July 2016 3,144 0 View

How do I reduce the self-fluorescence of cells of Ehrlich ascites carcinoma while performing an assay of DCFH-DA, in flow cytometry?

13 April 2013 8,401 6 View

Blood Perfusion during thermal stress

How does blood perfusion change during hyperthermia and hypothermia? What mathematical expressions exist for these phenomena? I would like indications of research about the subject. Thank you!

01 January 1970 6,395 2 View

I have developed a GPU parallelized Matlab code. I need to use NCI(supercomputer facilities) to speed up. Do I need to write any GPU script?

I have developed a GPU parallelized Matlab code. I need to use NCI(National computational infrastructure) to speed up the simulation. Do I need to write any special GPU script rater than CPU...

11 January 2021 9,454 3 View

CUDA Out Of Memory (OOM) error while using GPU?

I'm training a neural network (E3D-LSTM) for spatio temporal video prediction. It's quite a big network that has millions of parameters. The model is training perfectly when using my 12 CPU cores,...

25 December 2020 1,229 3 View

What are some Applications usecases that scale on application level metrics and not cpu or memory?

Can someone tell me use cases that uses multiple combination of custom metrics to scale or combination of custom and system metrics? when it comes to scaling in Kubernetes, we rely on metrics....

11 October 2020 4,129 4 View

How to run a Hamiltonian Replica Exchange Molecular Dynamics in the Gromacs patched with plumed?

Dear all, I'm trying to employ replica-exchange with solute tempering 2 (REST2) in a system, but I have performance issues in the calculations. I'm using openmpi-3.0.0 and gromacs 2019.4 patched...

29 September 2020 2,963 1 View

How to select a suitable method for association mapping ?

In analysis for association mapping I have used mutliple methods like MLM, CMLM, FarmCPU. Farm CPU yielded most of the markers fitting the expected distribution of p-values with a few markers...

25 September 2020 4,179 1 View

How to use all of CPU capacity for Analysis in Ansys mechanical Apdl?

Hello. Im using Ansys mechanical Apdl for timehistory analysis. I want to use all of Cpu's cores in this process. Help me please to do that with your guidance. #ansys #cpu_capacity

23 June 2020 7,600 3 View

Is it true more CPU core is better for deep learning?

I just started to learn the deep learning in my free time. I was hoping to buy a laptop where I want to implement some small(alexnet) to medium(GoogleNet) networks maybe something bigger. I...

22 June 2020 5,504 29 View

Any help on running GAMESS for quantum mechanical calculations?

Respected Scientists and Researchers, I am completely new to the field of computational chemistry. I am trying to run GAMESS by taking help of some tutorial videos available on internet. However...

11 June 2020 5,094 1 View

How to compare the computational efforts when the computations to be compared are being run on different computers with different CPU and RAM?

Consider you have two computational methods to solve a specific problem (also the corresponding software) and want to see which method is more efficient for the specific problem. Is it possible to...

31 May 2020 7,512 6 View

How to run NAMD on GPU?

31 May 2020 8,616 2 View