Immunohistochemistry of skin tumors in mice?

Hello everyone! I am going to perform immunohistochemistry on skin sections from mice. I am looking for a good antibody to look at pMEK and to look at BRAF(V600E) in mouse. Do you have any advice?...

08 September 2017 9,926 0 View

Immunohistochemistry protocol for mice tail?

Hello! Does anyone know a good protocol to perform immunohistochemistry on the skin of mice tail? Thank you very much in advance :)

01 February 2017 8,639 2 View

Fibroblastic reticular cells from mouse lymph nodes?

Good morning everyone!In my PhD project I will have to isolate fibroblastic reticular cells from mice lymph nodes, have you ever used some protocols to isolate them and then culture them?Thank you...

09 October 2016 9,566 3 View

A375 cell line?

Hi everybody! I am working in my internship with the human melanoma A375 cell line, I'd like to ask you if the genome of A375 cell line has been sequenced and where I can find data about...

04 May 2016 5,334 4 View

IWP-2 inhibitor

01 February 2016 7,636 4 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

No title

02 March 2021 3,060 3 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View