How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

No title

03 March 2021 5,360 2 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

The procedure compute?

02 March 2021 5,204 3 View

How to get TOPMED reference panel ( or south asian specific) for the imputation in Michigan Imputation Server?

Hi All, I am staring into imputation of variants for the genome wide scanning array in past weeks. Though I am able to do imputation using 1000GP3 panel using Impute2 for my dataset, I...

21 August 2020 2,719 1 View

Why we got more than one repeat for each primer used in NGS applied for DNA sequencing from each sample of date palm tree ?

Our project objective is to investigate the genetic diversity of Palestinian date palm, cultivars. 6 primers were ordered, these primers were designed specifically for NGS analysis. DNA was...

06 February 2020 695 2 View

Why we got more than one repeat for each primer used in NGS applied for DNA sequencing from each sample of date palm tree ?

Our project objective is to investigate the genetic diversity of Palestinian date palm, cultivars. 6 primers were ordered, these primers were designed specifically for NGS analysis. DNA was...

01 February 2020 2,733 1 View

How we can determined the composition of oil palm trunk sap?

How we can determined the composition of oil palm trunk sap? I mean what type of ingredients are present in sap and what are the ingredient percentage in oil palm trunk sap

01 February 2020 5,352 5 View

Why the biodiesel is not used commercially in India?

The problem is we couldn't even produce oil for our own citizen's needs. To meet the needs we are importing oil from other countries. In such situation we couldn't produce biodiesel from edible...

14 January 2020 6,268 5 View

How To Convert Snp haplotype Data Into 0,1 Matrix? Which software or package is better?

Hi everybody I want to convert HapMap Snp haplotype Data Into 0,1 Matrix so that I can use it in other works. I appreciate your help. Best regards

11 December 2019 414 3 View

Can I use the haploview to test linkage disequilbrium for around 10 SNPs?

I would like to perform linkage disequilibrium test for a number of SNPs. I am absolutely new to this field and would like to know what input file should be taken for my SNPs. I also would like to...

11 December 2019 8,416 2 View

How To Convert Snp haplotype Data Into 0,1 Matrix? Which software or package is better?

Hi everybody I want to convert HapMap Snp haplotype Data Into 0,1 Matrix so that I can use it in other works. I appreciate your help. Best regards

02 December 2019 3,312 4 View

What is the easier way to obtain Maize HapMap file?

I've been using Zea_mays.B73_RefGen_v4.dna.toplevel as reference genome for my analysis. At the same time, I need to use maize HapMap file? Does anyone used B74 RefGenv4 and HapMap3? I'm having...

08 September 2019 2,712 3 View

What is the easier way to obtain Maize HapMap file?

I've been using Zea_mays.B73_RefGen_v4.dna.toplevel as reference genome for my analysis. At the same time, I need to use maize HapMap file? Does anyone used B74 RefGenv4 and HapMap3? I'm having...

03 September 2019 9,906 3 View