DAPI signal loss on tissue section?

20 January 2022 1 430 Report

Hi all, I have stained my tissue section (cry-section) with DAPI but I am unable to observe stained nucleus in my sample.

I have done a few times of imaging on my sample (I can still see the fluorescence) and kept laser power low, and the samples that are using the same blocking buffer are still showing DAPI signal. Not sure what went wrong....

Your comments are highly appreciated!!

Shalini Sanyal

Kar Wei Chin - Is it a case of signal quenching - As in, slides that were initially showing the DAPI signal are not showing it anymore? You will still be able to see fluorescence in that case, but as mentioned; the signal will be very weak and/ missing from significant portions of your slides/samples.

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