Greetings everyone!

I am interested in using CUT&TAG to pull down transcription factors and have some questions about the cutting range of the technique. Specifically, I'm curious if the Tn5 transposase employed in CUT&TAG can cut RNA or if it only cuts DNA. Additionally, I'd like to know the cutting range of the Tn5 transposase. While I understand that it detects transcription factors and cuts accordingly, I am seeking more information on the length of the resulting fragments and whether this aspect can be controlled.

If anyone has experience using the CUT&Tag Kit from Active Motif and could provide me with answers to these questions, I would greatly appreciate it.

Thank you in advance for your assistance!

Best regards,

Anan