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10 November 2018 916 0 View

What is the advantage of E6/E7 mRNA qRT-PCR assay for HR-HPVs over APTIMA HPV Assay in cervcal cancer screening?

08 September 2018 3,857 2 View

Systematic review Vs Scoping review?

Dear RG users, Is there a methodological difference between scoping review and systematic review? What is the clear boundary of these review types?

06 July 2018 8,423 10 View

Factors to be considered while working with a diagnostic marker ?

According to the latest publication on nature (https://www.nature.com/subjects/diagnostic-markers), a diagnostic marker is defines as 'a biological parameter that aid the diagnosis of a disease'....

05 June 2018 6,404 0 View

SPSS vs Stata?

SPSS and Stata are apparently useful for epidemiological studies despite personal preferences. What is really the basic difference between these two packages? Which one is more convenient and...

04 May 2018 532 0 View

Which type of citation manager is use friendly and freely available?

I usually use EndNote to mange citations. Recently I have learned that Mendeley, Zotero and Reference manager tools are also widely applicable in the scientific world. I need experience from...

03 April 2018 4,053 0 View

How viruses evolved?

I have learned from different literature is that the origin of viruses is not well known though there are different theories on the subject. I am looking explanations from RG users on current...

02 March 2018 2,576 5 View

High levels of antibiotic resistance found worldwide, new data shows. How this could be contained?

01 February 2018 4,463 8 View

How can we find a suitable journal to publish our research paper?

What are the most important tips that we should consider to find suitable journal for our paper?

01 February 2018 3,113 8 View

What is the meaning of Odds ratio, Risk ratio and Risk difference and how they are interprated in epidemiological studies?

Dear RG users, I am wondering suggestions from colleagues; what is the clear boundary between OR and RR? How can I easily understand and interprate these epidemiological tools? Reflections are...

31 December 2017 9,723 2 View

Which of the Covid-19 Vaccines would elicit the best immunoprotection and why?

I want to know which of the available Covid-19 Vaccines would elicit the best immunological response and why

02 March 2021 1,388 3 View

Experimental Design: How deal with pseudreplicates with immunological and clinical-chemical paramaeters?

Hello everybody, I am investigating the influence of a toxinon various clinical-chemical and haematological parameters in fish. I have three treatments (control, low dose, high dose) in my...

24 February 2021 1,243 1 View

Looking for advices and tips to improve the staining and the gating strategy in human blood?

Hi, I am staining a human blood with CD19, CD3,CD14 and CD16. My gating strategy in FlowJo goes like this: - First, I gate a general population SSC-A VS FSC-A => Then gate for single cells, =>...

21 February 2021 5,724 4 View

Can you polarize Jurkat cells into different T helper subsets?

I am wondering if anyone has had success with in vitro polarization (via cytokines) of Jurkat cells. My goal is to understand the affect of a drug on Treg induction and differentiation. I...

12 January 2021 3,606 2 View

Can only the spike protein of the virus provide immunity?

Virology, microbiology, immunology, vaccine

21 December 2020 424 16 View

Can somebody recommend me a decent textbook to learn more about signaling pathways and their mechanistic principles in general?

I studied biochemistry and molecular biology in my bachelors and Immunology in my masters. I never came across a suitable course for general signaling pathways and principles. My interest is...

13 December 2020 5,690 5 View

Is it possible to extract DNA or RNA from paraffinized biopsies ?

My lab have some skin biopsies that were previously conserved in paraffine , and i wanted to know if it is possible to extract DNA or RNA from them.

06 December 2020 7,964 6 View

Could the desmin mutation in myofibrillar myopathy be caused by an infection?

A patient with desminopathy (mutation Thr341Pro DES in a heterozygous state) with the progression of the disease has a decrease in taste and smell, immunosuppression, and an increase in IgA in the...

02 November 2020 5,886 6 View

Whis HLA-A2 TAAs are best for DC pulsation and CD8+ cytotoxic T cell induction?

When looking to establish Ag-specific CD8 cytotoxic T cells with autologous dendritic cells, there are numerous tumor-associated Ags to choose from. Does anybody know which Ags give the best...

27 October 2020 2,068 2 View

Resuspending cell pellet after ficoll centrifugation?

In my current project I am planning to isolate mononuclear cells from peritoneal ascites of cancer patients using ficoll centrifugation. However, following isolation of the mononuclear cell layer,...

26 October 2020 5,966 4 View