I know that with estrogens it is advised to not have phenol red in your media due to its interference. I am planning on using IGF-1 and HGF (two steroid hormones) as treatments in vitro, and want to know whether anyone has any experience as to whether phenol red in media will interfere with my results. Have not found a conclusive answer either way online, and phenol red free media does seem to be mentioned in any of the literature I have come across. Thanks for any assistance.
Zvi,
Yes sir. That is what I am asking about. I have worked with estradiol in the past and know that you are suppose to use dextran charcoal on your media, but I am not sure if this extra step is necessary for IGF-1 and HGF. I do not believe it is, as I have not read any papers dealing with these treatments that have stripped their media in any way.
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