Joseph Wolf

6 Questions 4 Answers 0 Followers

Questions related from Joseph Wolf

Healthy cell comparison for breast cancer and prostate cancer?

I am trying to find the most acceptable healthy cell line to compare drug cytotoxicity against breast cancer and prostate cancer cell lines in vitro. Any assistance would be appreciated.

19 October 2020 3,889 3 View

Protein Bands Stopped Showing Up on Membrane?

I am conducting a Western Blot probing for CD133 and GAPDH. I use BioRad Stain-free Gels (any kD) and their turboblot accessory with their nitrocellulose mini membranes. My protocol is as...

13 January 2020 1,354 0 View

Curious if anyone has seen interference from phenol red when doing IGF-1 or HGF treatments in vitro?

I know that with estrogens it is advised to not have phenol red in your media due to its interference. I am planning on using IGF-1 and HGF (two steroid hormones) as treatments in vitro, and want...

19 December 2018 3,897 2 View

Does anyone have a protocol for using dextran-coated charcoal to strip FBS?

I have dextran-coated charcoal ( C6241 Sigma) and the protocols I have found require that the charcoal be coated and that solution is then used for the stripping. The problem is that that the...

22 July 2018 8,280 0 View

How to sterilize 17-beta estradiol?

I am planning on making working solutions of 17-beta estradiol in DMSO. The drug has been exposed outside of the culture hood. My studies will last up to 7 days, so I am wondering if I should...

09 July 2018 7,961 4 View

Has anyone used the CellInk Inkredible plus 3D Printer?

I am wondering if the post-processing script will insert the M-commands to turn the air off and on, set offsets, retract printheads, etc. automatically and correctly when I slice an object for...

22 March 2018 2,781 1 View