Hi everybody,
Does someone have already tried to inject CTB in E14.5 hind limb embryonic muscle?
Do you have any estimation of the speed rate of CTB-488 nm in the organism at this stage=?
Many thanks
PL
Hello
See this links
Hope help you
Good Luck
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3763960/
http://www.sciencedirect.com/science/article/pii/S0092867414012975
http://www.cell.com/cell/abstract/S0092-8674(14)01297-5
It's a minimum of 100mm/day in the adult rat CNS. Not sure about embryos.
Hi everyone, I'm a researcher working with proteins and I have a question about performing GPC (size exclusion chromatography). Lysozyme is known to hydrolyze peptidoglycan, which is a component...
25 May 2024 5,979 3 View
I perform al alkylation reaction on an imdazole compound where the alkyl reagent (1-Iodo-Octane) was in excess. After the reaction was completed, I then performed an extraction, followed by...
24 May 2024 8,042 2 View
Hello, I'm exploring whether incorporating edge weights in a network affects the extent to which the network is partitioned into clusters of closely connected nodes. Specifically, I'm...
13 May 2024 5,078 0 View
Dear Editor, I'm shocked that you publicized some papers that are fake here see https://www.researchgate.net/scientific-contributions/Serge-Valentin-Pangou-2003482514 See also the pdf attached...
26 March 2024 7,871 2 View
I was told by a lab colleague that 'after purifying a protein with a his-tag using imidazole, it is crucial to quickly remove the imidazole. Keeping the protein in more than 200mM of imidazole is...
04 March 2024 4,893 1 View
Stereo-video surveys of demersal fish assemblages are widely used. A field and video‐annotation guide for baited remote underwa... Stereoscopic footages are used to assess fish-body size. A...
22 January 2024 6,253 0 View
The emissions multiplier product matrix (EMPM) is an extension of the multiplier product matrix (MPM) based on the input-output table that takes into account the CO2 emissions generated by...
15 January 2024 7,111 0 View
My sample's dissolved oxygen is decreasing dramatically.What can I do to keep it stable or increase it again?
14 January 2024 3,308 7 View
Hi all, Do you do DO calibration while the production is ongoing? Typically I don't do it. However, my recent data showed that offline pO2 is extremely high (approximately 300mmHg) but the...
22 December 2023 3,270 0 View
hello, can you advise me a good wargame for pedagogy and training in strategy and tactic. I am searching something a bit serious designed in research and open.
30 November 2023 9,208 3 View
Best method and number of embryos for extracting RNA from pig morulae?
21 July 2024 4,398 1 View
Hello everyone, I am currently working on a project involving spatial transcriptomics on E14 mouse embryos. We need to perform cryosectioning without fixing the embryos. Here is the protocol we...
10 July 2024 5,985 0 View
With Fentanyl already being detected in some countries in Europe, I believe many of us are not educated enough on special needs of injection drug users that use Fentanyl, due to its high strength...
08 July 2024 7,694 3 View
I will be administering 5-fluorouracil at a dosage of 50 mg/kg to mice as an I/P injection. Will someone please check my calculation? (Mw: 130.078 g/mol) Max volume to be administered to mice I/P...
23 June 2024 3,847 1 View
I am currently working on my bachelor thesis in the field of back injection molding of textiles made of polyester fibers. We have achieved quite good results with thermal treatments to restore the...
01 June 2024 2,457 3 View
Reference for BMEL cells : Inducible differentiation and morphogenesis of bipotential liver cell lines from wild-type mouse embryos Hélène Strick-Marchand, Mary C Weiss doi:...
29 May 2024 7,198 0 View
Dear all, Hoping for ideas / suggestions for unclogging metallic fluid / paste dispense nozzles. Currently we have a metallic dispense nozzle clogged with silver conductive adhesive. We have...
29 May 2024 9,364 1 View
Hello, I am working on the mouse lens, in which I have obtained lens fiber and epithelium at two-time points, E17 (embryonic day 17) and P2 (postnatal day 2) for two different mouse strains,...
29 May 2024 3,367 0 View
Is there a dye and method that could be effective for staining the mitochondria of isolated insect embryos from eggs for confocal microscopy imaging? We are having trouble finding an appropriate...
21 May 2024 4,033 4 View
Hello everyone, I'm administering subanesthetic doses of ketamine to mice (10 mg/kg; i.p.), and I noticed that their motor coordination becomes compromised for up to an hour following injection....
20 May 2024 811 4 View