CTAB solution is not dissolving?

More Fizzah Zulfiqar's questions See All

Gallium Antimonide (GaSb). Can anyone help me to find vendor (provider) to sell GaSb solar cell ?

I am searching of provider (GaSb), I only found this one (https://jxcrystals.com/wp/about/). Can anyone help me?

21 February 2021 1,945 2 View

Why DNA is isolated along with RNA extraction?

I am using CTAB method for RNA extraction. However, during RNA extraction DNA also isolated and DNase treatment lowers the quantity of RNA. Kindly let me know the reason why DNA is coming along...

23 December 2020 9,627 4 View

Need Data on Following Variables for Given Countries?

Variables: Money , Banking Assets, Banking Loans, Industrial Production Index, Supply Chain Managers Index Countries: Pakistan, India, Bangladesh, USA, UK, UAE, and China Time: As much as possible

25 November 2020 4,206 2 View

PhD Thesis Idea on Econometrics?

Would you please suggest PhD Thesis Idea on Econometrics with 3 Gaps.

23 February 2020 464 4 View

Arc shaped heat source process examples?

Hello everyone, I am looking for some examples in which line/arc-shaped heat sources can be used. For example, the laser sintering process used a moving point heat source. In a similar way, I am...

22 December 2019 8,820 2 View

Genomic sequence amplification???

I am uploading 2kb sequence files of three genes any expert can help me to analyse these sequences and designed suitable primers for me as i have tried so many times before but i failed to get 2kb...

20 September 2019 340 3 View

Genomic sequence amplification???

I want to amplify the 5 prime upstream sequence up to 2kb, of genes, after several attempts i did not got specific primers or some suitable amplifying condition to get that sequence. Please...

13 September 2019 4,806 4 View

Calculation of anthocyanins in initial extracting sample?

I measure anthocyanin content in my samples. I measured the absorbance and put the values in equation. I am confused about the unit of of concentration. it should be mg/L? the initial...

05 September 2018 9,444 3 View

What are the superior properties which can only be seen/experienced in CNTs but not in Graphene, Graphene oxide or reduced graphene oxide?

Since graphene discovered (2004) later then CNTs (1991) it surpassed the CNTs in respect of many properties like adsorption and chemical catalysis due to high surface area available in case of...

07 August 2018 8,702 5 View

How to do one way ANOVA when one sample is larger than the other?

I want to compare the means of teachers and students perceptions collected from two likert scale questionnaires. I have 112 teacher respondents and 60 student respondents. Should I use all the...

26 February 2018 192 7 View

Is there anyone who is good with DNA extraction from hops?

Hi I extracted the DNA using the CTAB method and purified it with a kit, then I get this band like in the picture below. I am desperate, what can I do!

16 February 2021 5,508 3 View

How to remove colour from DNA pellet during DNA extraction?

I am working with Amaranth accessions which are green to red in color and contain high phenolic compounds. Using the CTAB method with the slight modification I am trying to get high-quality DNA so...

18 January 2021 3,578 1 View

How to Extract DNA from dried rhizomes, wood, galls etc. stored for a long time?

Dear Respectable Scientists, I have been trying to extract DNA from above mentioned sources using different CTAB/SDS Buffer Compositions and purifying agents but I am not able to extract DNA from...

12 January 2021 5,899 8 View

Why am I unable to coat oleate-capped NaCeF4:Gd,Tb nanoparticles with mesoporous silica shell?

My oleate-capped NaCeF4:Gd,Tb NPs have been synthesized via the thermal decomposition method. 1mmol of NPs has been dispersed in 20 ml of cyclohexane. I have tried dissolving 1 ml of NP in...

01 December 2020 4,713 2 View

Genomic DNA Exteraction from Asp nidulans?

Hi All, I have several trials for few months to extract good genomic DNA from some cultures of Asp. nidulans, most of them worked with CTAB extraction from freez-dried mycelium (30-40-60 hrs old)...

21 October 2020 6,030 3 View

Does the molecular weight of PVP matter in CTAB buffer for DNA and RNA extraction?

I am preparing CTAB buffer and I read that addition of PVP (Polyvinylpyrrolidone) helps to remove phenolic compounds. Most of the times in protocols there is no additional information about...

05 September 2020 7,488 4 View

What is the effect of Ascorbic acid in the synthesis of Au nanorods?

I prepared seed solution with HAuCl, CTAB, and NaBH4, after this growth solution with HAuCl4, CTAB, AgNO3, and Ascorbic acid. In the end step, I added Ascorbic acid in growth solution and solution...

17 August 2020 7,506 3 View

How much time is required to use TissueLyser LT ?

I am using TissueLyser LT to extract DNA from plant tissue ( without using liquid nitrogen cause it's not available ) but I do not know how much time is required? I did this in 6 minutes at 50HZ...

15 August 2020 9,270 7 View

How to purify the genomic DNA isolate that has been eluted by TE buffer?

I have done the procedure of DNA extraction using CTAB method. but the elecrophoresis result visualization showing that the isolate contains a lot of contaminant. Thank you.

20 July 2020 6,346 2 View

How to improve DNA quality for whole genome sequencing?

I am extracting DNA for bulking and sequencing. A260/280 of samples range from 1.90 - 2.05, and the values are higher than A260/230. After RNAse treatment, A260/280 ranged from 1.85 - 1.96....

29 April 2020 8,769 6 View

Isiaka Muhammad

Hi @Fizzah,

Did you try heating at 65 degrees on a magnetic stirrer? It should dissolve in this way.

Fizzah Zulfiqar

Isiaka Muhammad after that do I need to autoclave the solution? I am using CTAB solution for making RNA isolation extraction buffer.

Yes you'll need to autoclave the solution

Irina Kalish

According to the MSDS: CTAB's solubility in water is 36.4 g/l at 20 °C (68 °F) - completely soluble. It shouldn't require anything special. Do you have a fresh CTAB?