Isiaka Muhammad

4 Questions 12 Answers 0 Followers

Questions related from Isiaka Muhammad

Why do I have a low protein yield after an on-column gst cleavage elution?

Hi, I'm looking for a possible reason why I got low yield protein from a Pierce GST spin-column (Thermofisher) after an ON incubation with GST-cleavage enzyme and concentrating in an amicon...

25 March 2021 3,285 2 View

Why bacteria gene was found in a ligation after transformation analysis?

Hi everyone, I am doing a conventional cloning ligation procedure using double digestion (BamHI and EcoRI) using pGEX-6P-1 vector with my purified PCR product of 393bp. After ligation in 6:1...

05 August 2020 5,448 6 View

Cutsmart buffer and fastdigest green buffer, which one is compatible in double digestion with BamHI(fastdgiest) and EcoRI(HF) ?

I want to double restrict PCR product with fastdigest BamHI and NEB EcoRI (HF). Is cutsmart buffer or fastdigest Green buffer compatible for the double digestion?

06 August 2019 9,663 3 View

Is there a way to cleave a GST tagged proteins using pDEST15 from gateway system. I realised the pDEST didn’t have Protease cleavage site?

Is there a way to cleave a GST tagged protein expressed using pDEST15 from a gateway system? I lately realised the pDEST15 dont have Protease cleavage site. I expressed my protein in BL21-AI...

01 November 2018 2,809 6 View